Origin:
Derived from the ovary of an adult Chinese hamster
Species:
Cricetulus griseus (hamster, Chinese)
Tissue:
Ovary
Morphology:
Epithelial
Properties:
Adherent
Cytogenic data:
Stemline number is hypodiploid
Medium:
Ham's F12 + 10% FBS
Subculture:
1:4 to 1:10 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37 degree C
Freezing Medium:
Complete culture medium supplemented with 5% (v/v) DMSO
Biosafety Level:
I Sterility:
Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens:
HIV: Negative Hepatitis B: Negative Hepatitis C: Negative