RPMI-8226 cells

Derived from B lymphocytes

Species:

Homo sapiens

Tissue:

Blood

Morphology:

Lymphoblast

Properties:

Suspension

Cytogenic data:

Unstable karyotype in triploid

Patient:

Male, 61 yrs of age

Medium:

RPMI1640 + 10% FBS

Subculture:

Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 5 X 10⁵ viable cells/ml. Do not allow the cell density to exceed 2 to 3 X 10⁶ cells/ml. Culture cells at 5% CO₂, 37C.

Medium Renewal:

Every 2 to 3 days.

Freezing Medium:

Complete culture medium supplemented with 5% (v/v) DMSO

Biosafety Level: