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Plasmacytoma; myeloma Origin: Derived from B lymphocytes Species:Homo sapiens Tissue:Blood Morphology:Lymphoblast Properties:Suspension Cytogenic data:Unstable karyotype in triploid Patient:Male, 61 yrs of age Medium:RPMI1640 + 10% FBS Subculture:Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 5 X 105 viable cells/ml. Do not allow the cell density to exceed 2 to 3 X 106 cells/ml. Culture cells at 5% CO2, 37C. Medium Renewal:Every 2 to 3 days. Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Isoenzymes: G6PD, A DNA Profile (STR): Amelogenin: X, Y; CSF1PO: 12; D13S317: 11; D16S539: 9; D5S818: 11, 13; D7S820: 9, 10; THO1: 8; TPOX: 8, 11; vWA: 16, 18 Related cells by Organ: CCRF-CEM K562 LP-1 MOLM-13 RPMI-8226 U937 RAW 264.7
Derived from B lymphocytes Species:Homo sapiens Tissue:Blood Morphology:Lymphoblast Properties:Suspension Cytogenic data:Unstable karyotype in triploid Patient:Male, 61 yrs of age Medium:RPMI1640 + 10% FBS Subculture:Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 5 X 105 viable cells/ml. Do not allow the cell density to exceed 2 to 3 X 106 cells/ml. Culture cells at 5% CO2, 37C. Medium Renewal:Every 2 to 3 days. Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Isoenzymes: G6PD, A DNA Profile (STR): Amelogenin: X, Y; CSF1PO: 12; D13S317: 11; D16S539: 9; D5S818: 11, 13; D7S820: 9, 10; THO1: 8; TPOX: 8, 11; vWA: 16, 18 Related cells by Organ: CCRF-CEM K562 LP-1 MOLM-13 RPMI-8226 U937 RAW 264.7
Homo sapiens Tissue:Blood Morphology:Lymphoblast Properties:Suspension Cytogenic data:Unstable karyotype in triploid Patient:Male, 61 yrs of age Medium:RPMI1640 + 10% FBS Subculture:Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 5 X 105 viable cells/ml. Do not allow the cell density to exceed 2 to 3 X 106 cells/ml. Culture cells at 5% CO2, 37C. Medium Renewal:Every 2 to 3 days. Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Isoenzymes: G6PD, A DNA Profile (STR): Amelogenin: X, Y; CSF1PO: 12; D13S317: 11; D16S539: 9; D5S818: 11, 13; D7S820: 9, 10; THO1: 8; TPOX: 8, 11; vWA: 16, 18 Related cells by Organ: CCRF-CEM K562 LP-1 MOLM-13 RPMI-8226 U937 RAW 264.7
Blood Morphology:Lymphoblast Properties:Suspension Cytogenic data:Unstable karyotype in triploid Patient:Male, 61 yrs of age Medium:RPMI1640 + 10% FBS Subculture:Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 5 X 105 viable cells/ml. Do not allow the cell density to exceed 2 to 3 X 106 cells/ml. Culture cells at 5% CO2, 37C. Medium Renewal:Every 2 to 3 days. Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Isoenzymes: G6PD, A DNA Profile (STR): Amelogenin: X, Y; CSF1PO: 12; D13S317: 11; D16S539: 9; D5S818: 11, 13; D7S820: 9, 10; THO1: 8; TPOX: 8, 11; vWA: 16, 18 Related cells by Organ: CCRF-CEM K562 LP-1 MOLM-13 RPMI-8226 U937 RAW 264.7
Lymphoblast Properties:Suspension Cytogenic data:Unstable karyotype in triploid Patient:Male, 61 yrs of age Medium:RPMI1640 + 10% FBS Subculture:Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 5 X 105 viable cells/ml. Do not allow the cell density to exceed 2 to 3 X 106 cells/ml. Culture cells at 5% CO2, 37C. Medium Renewal:Every 2 to 3 days. Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Isoenzymes: G6PD, A DNA Profile (STR): Amelogenin: X, Y; CSF1PO: 12; D13S317: 11; D16S539: 9; D5S818: 11, 13; D7S820: 9, 10; THO1: 8; TPOX: 8, 11; vWA: 16, 18 Related cells by Organ: CCRF-CEM K562 LP-1 MOLM-13 RPMI-8226 U937 RAW 264.7
Suspension Cytogenic data:Unstable karyotype in triploid Patient:Male, 61 yrs of age Medium:RPMI1640 + 10% FBS Subculture:Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 5 X 105 viable cells/ml. Do not allow the cell density to exceed 2 to 3 X 106 cells/ml. Culture cells at 5% CO2, 37C. Medium Renewal:Every 2 to 3 days. Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Isoenzymes: G6PD, A DNA Profile (STR): Amelogenin: X, Y; CSF1PO: 12; D13S317: 11; D16S539: 9; D5S818: 11, 13; D7S820: 9, 10; THO1: 8; TPOX: 8, 11; vWA: 16, 18 Related cells by Organ: CCRF-CEM K562 LP-1 MOLM-13 RPMI-8226 U937 RAW 264.7
Unstable karyotype in triploid Patient:Male, 61 yrs of age Medium:RPMI1640 + 10% FBS Subculture:Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 5 X 105 viable cells/ml. Do not allow the cell density to exceed 2 to 3 X 106 cells/ml. Culture cells at 5% CO2, 37C. Medium Renewal:Every 2 to 3 days. Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Isoenzymes: G6PD, A DNA Profile (STR): Amelogenin: X, Y; CSF1PO: 12; D13S317: 11; D16S539: 9; D5S818: 11, 13; D7S820: 9, 10; THO1: 8; TPOX: 8, 11; vWA: 16, 18 Related cells by Organ: CCRF-CEM K562 LP-1 MOLM-13 RPMI-8226 U937 RAW 264.7
Male, 61 yrs of age Medium:RPMI1640 + 10% FBS Subculture:Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 5 X 105 viable cells/ml. Do not allow the cell density to exceed 2 to 3 X 106 cells/ml. Culture cells at 5% CO2, 37C. Medium Renewal:Every 2 to 3 days. Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Isoenzymes: G6PD, A DNA Profile (STR): Amelogenin: X, Y; CSF1PO: 12; D13S317: 11; D16S539: 9; D5S818: 11, 13; D7S820: 9, 10; THO1: 8; TPOX: 8, 11; vWA: 16, 18 Related cells by Organ: CCRF-CEM K562 LP-1 MOLM-13 RPMI-8226 U937 RAW 264.7
RPMI1640 + 10% FBS Subculture:Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 5 X 105 viable cells/ml. Do not allow the cell density to exceed 2 to 3 X 106 cells/ml. Culture cells at 5% CO2, 37C. Medium Renewal:Every 2 to 3 days. Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Isoenzymes: G6PD, A DNA Profile (STR): Amelogenin: X, Y; CSF1PO: 12; D13S317: 11; D16S539: 9; D5S818: 11, 13; D7S820: 9, 10; THO1: 8; TPOX: 8, 11; vWA: 16, 18 Related cells by Organ: CCRF-CEM K562 LP-1 MOLM-13 RPMI-8226 U937 RAW 264.7
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 5 X 105 viable cells/ml. Do not allow the cell density to exceed 2 to 3 X 106 cells/ml. Culture cells at 5% CO2, 37C. Medium Renewal:Every 2 to 3 days. Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Isoenzymes: G6PD, A DNA Profile (STR): Amelogenin: X, Y; CSF1PO: 12; D13S317: 11; D16S539: 9; D5S818: 11, 13; D7S820: 9, 10; THO1: 8; TPOX: 8, 11; vWA: 16, 18 Related cells by Organ: CCRF-CEM K562 LP-1 MOLM-13 RPMI-8226 U937 RAW 264.7
Every 2 to 3 days. Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Isoenzymes: G6PD, A DNA Profile (STR): Amelogenin: X, Y; CSF1PO: 12; D13S317: 11; D16S539: 9; D5S818: 11, 13; D7S820: 9, 10; THO1: 8; TPOX: 8, 11; vWA: 16, 18 Related cells by Organ: CCRF-CEM K562 LP-1 MOLM-13 RPMI-8226 U937 RAW 264.7
Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Isoenzymes: G6PD, A DNA Profile (STR): Amelogenin: X, Y; CSF1PO: 12; D13S317: 11; D16S539: 9; D5S818: 11, 13; D7S820: 9, 10; THO1: 8; TPOX: 8, 11; vWA: 16, 18 Related cells by Organ: CCRF-CEM K562 LP-1 MOLM-13 RPMI-8226 U937 RAW 264.7
I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Isoenzymes: G6PD, A DNA Profile (STR): Amelogenin: X, Y; CSF1PO: 12; D13S317: 11; D16S539: 9; D5S818: 11, 13; D7S820: 9, 10; THO1: 8; TPOX: 8, 11; vWA: 16, 18 Related cells by Organ: CCRF-CEM K562 LP-1 MOLM-13 RPMI-8226 U937 RAW 264.7
Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Isoenzymes: G6PD, A DNA Profile (STR): Amelogenin: X, Y; CSF1PO: 12; D13S317: 11; D16S539: 9; D5S818: 11, 13; D7S820: 9, 10; THO1: 8; TPOX: 8, 11; vWA: 16, 18 Related cells by Organ: CCRF-CEM K562 LP-1 MOLM-13 RPMI-8226 U937 RAW 264.7
Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Isoenzymes: G6PD, A DNA Profile (STR): Amelogenin: X, Y; CSF1PO: 12; D13S317: 11; D16S539: 9; D5S818: 11, 13; D7S820: 9, 10; THO1: 8; TPOX: 8, 11; vWA: 16, 18 Related cells by Organ: CCRF-CEM K562 LP-1 MOLM-13 RPMI-8226 U937 RAW 264.7
Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Isoenzymes: G6PD, A DNA Profile (STR): Amelogenin: X, Y; CSF1PO: 12; D13S317: 11; D16S539: 9; D5S818: 11, 13; D7S820: 9, 10; THO1: 8; TPOX: 8, 11; vWA: 16, 18 Related cells by Organ: CCRF-CEM K562 LP-1 MOLM-13 RPMI-8226 U937 RAW 264.7
HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Isoenzymes: G6PD, A DNA Profile (STR): Amelogenin: X, Y; CSF1PO: 12; D13S317: 11; D16S539: 9; D5S818: 11, 13; D7S820: 9, 10; THO1: 8; TPOX: 8, 11; vWA: 16, 18 Related cells by Organ: CCRF-CEM K562 LP-1 MOLM-13 RPMI-8226 U937 RAW 264.7
Hepatitis B: Negative Hepatitis C: Negative Isoenzymes: G6PD, A DNA Profile (STR): Amelogenin: X, Y; CSF1PO: 12; D13S317: 11; D16S539: 9; D5S818: 11, 13; D7S820: 9, 10; THO1: 8; TPOX: 8, 11; vWA: 16, 18 Related cells by Organ: CCRF-CEM K562 LP-1 MOLM-13 RPMI-8226 U937 RAW 264.7
Hepatitis C: Negative Isoenzymes: G6PD, A DNA Profile (STR): Amelogenin: X, Y; CSF1PO: 12; D13S317: 11; D16S539: 9; D5S818: 11, 13; D7S820: 9, 10; THO1: 8; TPOX: 8, 11; vWA: 16, 18 Related cells by Organ: CCRF-CEM K562 LP-1 MOLM-13 RPMI-8226 U937 RAW 264.7
CCRF-CEM
K562
LP-1
MOLM-13
RPMI-8226
U937
RAW 264.7
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