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Chronic myelogenous leukemia (CML) Origin: Established from pleural effusion Species:Homo sapiens Tissue:Blood Morphology:Lymphoblast Properties:Suspension Cytogenic data:The stemline chromosome number is triploid Patient:Female, 53 yrs of age Medium:IMDM +10% FBS Subculture:Cultures can be maintained by the addition or replacement of fresh medium. Start new cultures at 1 X 105 viable cells/ml. Subculture at 1 X 106 cells/ml. Culture cells at 5% CO2, 37C. Medium Renewal:Add fresh medium every 2 to 3 days. Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Isoenzymes: AK-1, 1; ES-D, 1; G6PD, B; GLO-I, 2; Me-2, 0; PGM1, 0; PGM3, 1 DNA Profile (STR): Amelogenin: X; CSF1PO: 9, 10; D13S317: 8; D16S539: 11, 12; D5S818: 11, 12; D7S820: 9, 11; THO1: 9.3; TPOX: 8, 9; vWA: 16 Mutations in K562 Cells Related cells by Organ: CCRF-CEM K562 LP-1 MOLM-13 RPMI-8226 U937 RAW 264.7
Established from pleural effusion Species:Homo sapiens Tissue:Blood Morphology:Lymphoblast Properties:Suspension Cytogenic data:The stemline chromosome number is triploid Patient:Female, 53 yrs of age Medium:IMDM +10% FBS Subculture:Cultures can be maintained by the addition or replacement of fresh medium. Start new cultures at 1 X 105 viable cells/ml. Subculture at 1 X 106 cells/ml. Culture cells at 5% CO2, 37C. Medium Renewal:Add fresh medium every 2 to 3 days. Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Isoenzymes: AK-1, 1; ES-D, 1; G6PD, B; GLO-I, 2; Me-2, 0; PGM1, 0; PGM3, 1 DNA Profile (STR): Amelogenin: X; CSF1PO: 9, 10; D13S317: 8; D16S539: 11, 12; D5S818: 11, 12; D7S820: 9, 11; THO1: 9.3; TPOX: 8, 9; vWA: 16 Mutations in K562 Cells Related cells by Organ: CCRF-CEM K562 LP-1 MOLM-13 RPMI-8226 U937 RAW 264.7
Homo sapiens Tissue:Blood Morphology:Lymphoblast Properties:Suspension Cytogenic data:The stemline chromosome number is triploid Patient:Female, 53 yrs of age Medium:IMDM +10% FBS Subculture:Cultures can be maintained by the addition or replacement of fresh medium. Start new cultures at 1 X 105 viable cells/ml. Subculture at 1 X 106 cells/ml. Culture cells at 5% CO2, 37C. Medium Renewal:Add fresh medium every 2 to 3 days. Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Isoenzymes: AK-1, 1; ES-D, 1; G6PD, B; GLO-I, 2; Me-2, 0; PGM1, 0; PGM3, 1 DNA Profile (STR): Amelogenin: X; CSF1PO: 9, 10; D13S317: 8; D16S539: 11, 12; D5S818: 11, 12; D7S820: 9, 11; THO1: 9.3; TPOX: 8, 9; vWA: 16 Mutations in K562 Cells Related cells by Organ: CCRF-CEM K562 LP-1 MOLM-13 RPMI-8226 U937 RAW 264.7
Blood Morphology:Lymphoblast Properties:Suspension Cytogenic data:The stemline chromosome number is triploid Patient:Female, 53 yrs of age Medium:IMDM +10% FBS Subculture:Cultures can be maintained by the addition or replacement of fresh medium. Start new cultures at 1 X 105 viable cells/ml. Subculture at 1 X 106 cells/ml. Culture cells at 5% CO2, 37C. Medium Renewal:Add fresh medium every 2 to 3 days. Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Isoenzymes: AK-1, 1; ES-D, 1; G6PD, B; GLO-I, 2; Me-2, 0; PGM1, 0; PGM3, 1 DNA Profile (STR): Amelogenin: X; CSF1PO: 9, 10; D13S317: 8; D16S539: 11, 12; D5S818: 11, 12; D7S820: 9, 11; THO1: 9.3; TPOX: 8, 9; vWA: 16 Mutations in K562 Cells Related cells by Organ: CCRF-CEM K562 LP-1 MOLM-13 RPMI-8226 U937 RAW 264.7
Lymphoblast Properties:Suspension Cytogenic data:The stemline chromosome number is triploid Patient:Female, 53 yrs of age Medium:IMDM +10% FBS Subculture:Cultures can be maintained by the addition or replacement of fresh medium. Start new cultures at 1 X 105 viable cells/ml. Subculture at 1 X 106 cells/ml. Culture cells at 5% CO2, 37C. Medium Renewal:Add fresh medium every 2 to 3 days. Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Isoenzymes: AK-1, 1; ES-D, 1; G6PD, B; GLO-I, 2; Me-2, 0; PGM1, 0; PGM3, 1 DNA Profile (STR): Amelogenin: X; CSF1PO: 9, 10; D13S317: 8; D16S539: 11, 12; D5S818: 11, 12; D7S820: 9, 11; THO1: 9.3; TPOX: 8, 9; vWA: 16 Mutations in K562 Cells Related cells by Organ: CCRF-CEM K562 LP-1 MOLM-13 RPMI-8226 U937 RAW 264.7
Suspension Cytogenic data:The stemline chromosome number is triploid Patient:Female, 53 yrs of age Medium:IMDM +10% FBS Subculture:Cultures can be maintained by the addition or replacement of fresh medium. Start new cultures at 1 X 105 viable cells/ml. Subculture at 1 X 106 cells/ml. Culture cells at 5% CO2, 37C. Medium Renewal:Add fresh medium every 2 to 3 days. Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Isoenzymes: AK-1, 1; ES-D, 1; G6PD, B; GLO-I, 2; Me-2, 0; PGM1, 0; PGM3, 1 DNA Profile (STR): Amelogenin: X; CSF1PO: 9, 10; D13S317: 8; D16S539: 11, 12; D5S818: 11, 12; D7S820: 9, 11; THO1: 9.3; TPOX: 8, 9; vWA: 16 Mutations in K562 Cells Related cells by Organ: CCRF-CEM K562 LP-1 MOLM-13 RPMI-8226 U937 RAW 264.7
The stemline chromosome number is triploid Patient:Female, 53 yrs of age Medium:IMDM +10% FBS Subculture:Cultures can be maintained by the addition or replacement of fresh medium. Start new cultures at 1 X 105 viable cells/ml. Subculture at 1 X 106 cells/ml. Culture cells at 5% CO2, 37C. Medium Renewal:Add fresh medium every 2 to 3 days. Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Isoenzymes: AK-1, 1; ES-D, 1; G6PD, B; GLO-I, 2; Me-2, 0; PGM1, 0; PGM3, 1 DNA Profile (STR): Amelogenin: X; CSF1PO: 9, 10; D13S317: 8; D16S539: 11, 12; D5S818: 11, 12; D7S820: 9, 11; THO1: 9.3; TPOX: 8, 9; vWA: 16 Mutations in K562 Cells Related cells by Organ: CCRF-CEM K562 LP-1 MOLM-13 RPMI-8226 U937 RAW 264.7
Female, 53 yrs of age Medium:IMDM +10% FBS Subculture:Cultures can be maintained by the addition or replacement of fresh medium. Start new cultures at 1 X 105 viable cells/ml. Subculture at 1 X 106 cells/ml. Culture cells at 5% CO2, 37C. Medium Renewal:Add fresh medium every 2 to 3 days. Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Isoenzymes: AK-1, 1; ES-D, 1; G6PD, B; GLO-I, 2; Me-2, 0; PGM1, 0; PGM3, 1 DNA Profile (STR): Amelogenin: X; CSF1PO: 9, 10; D13S317: 8; D16S539: 11, 12; D5S818: 11, 12; D7S820: 9, 11; THO1: 9.3; TPOX: 8, 9; vWA: 16 Mutations in K562 Cells Related cells by Organ: CCRF-CEM K562 LP-1 MOLM-13 RPMI-8226 U937 RAW 264.7
IMDM +10% FBS Subculture:Cultures can be maintained by the addition or replacement of fresh medium. Start new cultures at 1 X 105 viable cells/ml. Subculture at 1 X 106 cells/ml. Culture cells at 5% CO2, 37C. Medium Renewal:Add fresh medium every 2 to 3 days. Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Isoenzymes: AK-1, 1; ES-D, 1; G6PD, B; GLO-I, 2; Me-2, 0; PGM1, 0; PGM3, 1 DNA Profile (STR): Amelogenin: X; CSF1PO: 9, 10; D13S317: 8; D16S539: 11, 12; D5S818: 11, 12; D7S820: 9, 11; THO1: 9.3; TPOX: 8, 9; vWA: 16 Mutations in K562 Cells Related cells by Organ: CCRF-CEM K562 LP-1 MOLM-13 RPMI-8226 U937 RAW 264.7
Cultures can be maintained by the addition or replacement of fresh medium. Start new cultures at 1 X 105 viable cells/ml. Subculture at 1 X 106 cells/ml. Culture cells at 5% CO2, 37C. Medium Renewal:Add fresh medium every 2 to 3 days. Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Isoenzymes: AK-1, 1; ES-D, 1; G6PD, B; GLO-I, 2; Me-2, 0; PGM1, 0; PGM3, 1 DNA Profile (STR): Amelogenin: X; CSF1PO: 9, 10; D13S317: 8; D16S539: 11, 12; D5S818: 11, 12; D7S820: 9, 11; THO1: 9.3; TPOX: 8, 9; vWA: 16 Mutations in K562 Cells Related cells by Organ: CCRF-CEM K562 LP-1 MOLM-13 RPMI-8226 U937 RAW 264.7
Add fresh medium every 2 to 3 days. Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Isoenzymes: AK-1, 1; ES-D, 1; G6PD, B; GLO-I, 2; Me-2, 0; PGM1, 0; PGM3, 1 DNA Profile (STR): Amelogenin: X; CSF1PO: 9, 10; D13S317: 8; D16S539: 11, 12; D5S818: 11, 12; D7S820: 9, 11; THO1: 9.3; TPOX: 8, 9; vWA: 16 Mutations in K562 Cells Related cells by Organ: CCRF-CEM K562 LP-1 MOLM-13 RPMI-8226 U937 RAW 264.7
Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Isoenzymes: AK-1, 1; ES-D, 1; G6PD, B; GLO-I, 2; Me-2, 0; PGM1, 0; PGM3, 1 DNA Profile (STR): Amelogenin: X; CSF1PO: 9, 10; D13S317: 8; D16S539: 11, 12; D5S818: 11, 12; D7S820: 9, 11; THO1: 9.3; TPOX: 8, 9; vWA: 16 Mutations in K562 Cells Related cells by Organ: CCRF-CEM K562 LP-1 MOLM-13 RPMI-8226 U937 RAW 264.7
I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Isoenzymes: AK-1, 1; ES-D, 1; G6PD, B; GLO-I, 2; Me-2, 0; PGM1, 0; PGM3, 1 DNA Profile (STR): Amelogenin: X; CSF1PO: 9, 10; D13S317: 8; D16S539: 11, 12; D5S818: 11, 12; D7S820: 9, 11; THO1: 9.3; TPOX: 8, 9; vWA: 16 Mutations in K562 Cells Related cells by Organ: CCRF-CEM K562 LP-1 MOLM-13 RPMI-8226 U937 RAW 264.7
Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Isoenzymes: AK-1, 1; ES-D, 1; G6PD, B; GLO-I, 2; Me-2, 0; PGM1, 0; PGM3, 1 DNA Profile (STR): Amelogenin: X; CSF1PO: 9, 10; D13S317: 8; D16S539: 11, 12; D5S818: 11, 12; D7S820: 9, 11; THO1: 9.3; TPOX: 8, 9; vWA: 16 Mutations in K562 Cells Related cells by Organ: CCRF-CEM K562 LP-1 MOLM-13 RPMI-8226 U937 RAW 264.7
Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Isoenzymes: AK-1, 1; ES-D, 1; G6PD, B; GLO-I, 2; Me-2, 0; PGM1, 0; PGM3, 1 DNA Profile (STR): Amelogenin: X; CSF1PO: 9, 10; D13S317: 8; D16S539: 11, 12; D5S818: 11, 12; D7S820: 9, 11; THO1: 9.3; TPOX: 8, 9; vWA: 16 Mutations in K562 Cells Related cells by Organ: CCRF-CEM K562 LP-1 MOLM-13 RPMI-8226 U937 RAW 264.7
Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Isoenzymes: AK-1, 1; ES-D, 1; G6PD, B; GLO-I, 2; Me-2, 0; PGM1, 0; PGM3, 1 DNA Profile (STR): Amelogenin: X; CSF1PO: 9, 10; D13S317: 8; D16S539: 11, 12; D5S818: 11, 12; D7S820: 9, 11; THO1: 9.3; TPOX: 8, 9; vWA: 16 Mutations in K562 Cells Related cells by Organ: CCRF-CEM K562 LP-1 MOLM-13 RPMI-8226 U937 RAW 264.7
HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Isoenzymes: AK-1, 1; ES-D, 1; G6PD, B; GLO-I, 2; Me-2, 0; PGM1, 0; PGM3, 1 DNA Profile (STR): Amelogenin: X; CSF1PO: 9, 10; D13S317: 8; D16S539: 11, 12; D5S818: 11, 12; D7S820: 9, 11; THO1: 9.3; TPOX: 8, 9; vWA: 16 Mutations in K562 Cells Related cells by Organ: CCRF-CEM K562 LP-1 MOLM-13 RPMI-8226 U937 RAW 264.7
Hepatitis B: Negative Hepatitis C: Negative Isoenzymes: AK-1, 1; ES-D, 1; G6PD, B; GLO-I, 2; Me-2, 0; PGM1, 0; PGM3, 1 DNA Profile (STR): Amelogenin: X; CSF1PO: 9, 10; D13S317: 8; D16S539: 11, 12; D5S818: 11, 12; D7S820: 9, 11; THO1: 9.3; TPOX: 8, 9; vWA: 16 Mutations in K562 Cells Related cells by Organ: CCRF-CEM K562 LP-1 MOLM-13 RPMI-8226 U937 RAW 264.7
Hepatitis C: Negative Isoenzymes: AK-1, 1; ES-D, 1; G6PD, B; GLO-I, 2; Me-2, 0; PGM1, 0; PGM3, 1 DNA Profile (STR): Amelogenin: X; CSF1PO: 9, 10; D13S317: 8; D16S539: 11, 12; D5S818: 11, 12; D7S820: 9, 11; THO1: 9.3; TPOX: 8, 9; vWA: 16 Mutations in K562 Cells Related cells by Organ: CCRF-CEM K562 LP-1 MOLM-13 RPMI-8226 U937 RAW 264.7
Mutations in K562 Cells
K562
LP-1
MOLM-13
RPMI-8226
U937
RAW 264.7
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