Daudi cells

Disease:
Burkitt's lymphoma
Origin:
Established from the Burkitt lymphoma (left orbital biopsy) of a 16-year-old African boy in 1967; cells were described to be EBV-positive and to express mRNA for proto-oncogene BCL2
Species:
Homo sapiens
Tissue:
Peripheral blood
Morphology:
Lymphoblast
Properties:
Round single cells in suspension
Cytogenic data:
human near diploid karyotype with 20% polyploidy - 46(45-48)< 2n> XY/XXY, +7, -9, t(8; 14)(q24; q32)
Patient:
Male, Black, 16 yrs of age
Medium:
RPMI1640 + 10% FBS
Subculture:
maintain at 0.5-1.0 x 10^6 cells/ml; split ratio 1:2 to 1:4 every 2-4 days; cells may grow slowly initially; seed at 0.5-1 x 10^6 cells/ml; maximal cell density at 1.5x10^6 viable cells/mL; 5% CO2; 37 C
Freezing Medium:
Complete culture medium supplemented with 5% (v/v) DMSO
Biosafety Level:
II (Cells Contain HERPESVIRUS, EBV).
Sterility:
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
Pathogens:
HIV: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Isoenzymes: G6PD, B
DNA Profile (STR): Amelogenin: X, Y CSF1PO: 12; D13S317: 11, 12; D16S539: 10, 12; D5S818: 8, 13; D7S820: 8, 10; THO1: 6, 7; TPOX: 8, 11; vWA: 15, 17

Cell Lines