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large cell lymphoma; diffuse mixed histiocytic and lymphocytic lymphoma; follicular B cell lymphoma; B cell lymphoma
Established from the peritoneal effusion of a 43-year-old man with B-cell non-Hodgkin lymphoma (B-NHL), described at the time as diffuse, mixed small and large cell type; cell line carries EZH2 Y641N mutation; assigned to GCB-like lymphoma subtype (germinal center B-cell)
Homo sapiens Tissue:Peritoneal effusion Morphology:Lymphoblast-like Properties: Small single cells and in clusters, in suspension Cytogenic data: Human hyperdiploid karyotype with 2% polyploidy - 47(42-48)< 2n> X, -Y, +6, +7, del(4)(q23), del(6)(p21.3p22.2), i(6p), del(7)(q?22q?32), der(8)t(8; 9)(q24; p13), der(9)t(8; 19; 9)(q24; q13; p13), dup(11)(q24q25), t(14; 18)(q32; q21), der(22)t(?7; 22)(?q32; p11) - sideline with dic(8; 9)(q24; p13), ider(8)(q10)t(8; 9(q24; p13) - matches published karyotype - carries t(14; 18) effecting IGH-BCL2 fusion Patient: Male, Caucasian, 43 yrs of age Medium: RPMI-1640 Medium + 10% FBS Subculture: Cultures can be maintained by the addition of fresh medium. Seed cells at 8 X 104 to 3 x 10^5 cells/mL is recommended. Subculture when cell concentration is between 7 x 105 and 1 x 10^6 cells/mL; a certain amount of cell debris is always visible; A subcultivation ratio of 1:3 to 1:4 is recommended.5% CO2; 37C Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative DNA Profile (STR): D5S818: 12; D13S317: 12, 14; D7S820: 10; D16S539: 11, 12; vWA: 14, 17; THO1: 6, 9.3; TPOX: 11, 12; CSF1PO: 10; Amelogenin: X
Peritoneal effusion Morphology:Lymphoblast-like Properties: Small single cells and in clusters, in suspension Cytogenic data: Human hyperdiploid karyotype with 2% polyploidy - 47(42-48)< 2n> X, -Y, +6, +7, del(4)(q23), del(6)(p21.3p22.2), i(6p), del(7)(q?22q?32), der(8)t(8; 9)(q24; p13), der(9)t(8; 19; 9)(q24; q13; p13), dup(11)(q24q25), t(14; 18)(q32; q21), der(22)t(?7; 22)(?q32; p11) - sideline with dic(8; 9)(q24; p13), ider(8)(q10)t(8; 9(q24; p13) - matches published karyotype - carries t(14; 18) effecting IGH-BCL2 fusion Patient: Male, Caucasian, 43 yrs of age Medium: RPMI-1640 Medium + 10% FBS Subculture: Cultures can be maintained by the addition of fresh medium. Seed cells at 8 X 104 to 3 x 10^5 cells/mL is recommended. Subculture when cell concentration is between 7 x 105 and 1 x 10^6 cells/mL; a certain amount of cell debris is always visible; A subcultivation ratio of 1:3 to 1:4 is recommended.5% CO2; 37C Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative DNA Profile (STR): D5S818: 12; D13S317: 12, 14; D7S820: 10; D16S539: 11, 12; vWA: 14, 17; THO1: 6, 9.3; TPOX: 11, 12; CSF1PO: 10; Amelogenin: X
Lymphoblast-like Properties: Small single cells and in clusters, in suspension Cytogenic data: Human hyperdiploid karyotype with 2% polyploidy - 47(42-48)< 2n> X, -Y, +6, +7, del(4)(q23), del(6)(p21.3p22.2), i(6p), del(7)(q?22q?32), der(8)t(8; 9)(q24; p13), der(9)t(8; 19; 9)(q24; q13; p13), dup(11)(q24q25), t(14; 18)(q32; q21), der(22)t(?7; 22)(?q32; p11) - sideline with dic(8; 9)(q24; p13), ider(8)(q10)t(8; 9(q24; p13) - matches published karyotype - carries t(14; 18) effecting IGH-BCL2 fusion Patient: Male, Caucasian, 43 yrs of age Medium: RPMI-1640 Medium + 10% FBS Subculture: Cultures can be maintained by the addition of fresh medium. Seed cells at 8 X 104 to 3 x 10^5 cells/mL is recommended. Subculture when cell concentration is between 7 x 105 and 1 x 10^6 cells/mL; a certain amount of cell debris is always visible; A subcultivation ratio of 1:3 to 1:4 is recommended.5% CO2; 37C Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative DNA Profile (STR): D5S818: 12; D13S317: 12, 14; D7S820: 10; D16S539: 11, 12; vWA: 14, 17; THO1: 6, 9.3; TPOX: 11, 12; CSF1PO: 10; Amelogenin: X
Small single cells and in clusters, in suspension Cytogenic data: Human hyperdiploid karyotype with 2% polyploidy - 47(42-48)< 2n> X, -Y, +6, +7, del(4)(q23), del(6)(p21.3p22.2), i(6p), del(7)(q?22q?32), der(8)t(8; 9)(q24; p13), der(9)t(8; 19; 9)(q24; q13; p13), dup(11)(q24q25), t(14; 18)(q32; q21), der(22)t(?7; 22)(?q32; p11) - sideline with dic(8; 9)(q24; p13), ider(8)(q10)t(8; 9(q24; p13) - matches published karyotype - carries t(14; 18) effecting IGH-BCL2 fusion Patient: Male, Caucasian, 43 yrs of age Medium: RPMI-1640 Medium + 10% FBS Subculture: Cultures can be maintained by the addition of fresh medium. Seed cells at 8 X 104 to 3 x 10^5 cells/mL is recommended. Subculture when cell concentration is between 7 x 105 and 1 x 10^6 cells/mL; a certain amount of cell debris is always visible; A subcultivation ratio of 1:3 to 1:4 is recommended.5% CO2; 37C Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative DNA Profile (STR): D5S818: 12; D13S317: 12, 14; D7S820: 10; D16S539: 11, 12; vWA: 14, 17; THO1: 6, 9.3; TPOX: 11, 12; CSF1PO: 10; Amelogenin: X
Human hyperdiploid karyotype with 2% polyploidy - 47(42-48)< 2n> X, -Y, +6, +7, del(4)(q23), del(6)(p21.3p22.2), i(6p), del(7)(q?22q?32), der(8)t(8; 9)(q24; p13), der(9)t(8; 19; 9)(q24; q13; p13), dup(11)(q24q25), t(14; 18)(q32; q21), der(22)t(?7; 22)(?q32; p11) - sideline with dic(8; 9)(q24; p13), ider(8)(q10)t(8; 9(q24; p13) - matches published karyotype - carries t(14; 18) effecting IGH-BCL2 fusion Patient: Male, Caucasian, 43 yrs of age Medium: RPMI-1640 Medium + 10% FBS Subculture: Cultures can be maintained by the addition of fresh medium. Seed cells at 8 X 104 to 3 x 10^5 cells/mL is recommended. Subculture when cell concentration is between 7 x 105 and 1 x 10^6 cells/mL; a certain amount of cell debris is always visible; A subcultivation ratio of 1:3 to 1:4 is recommended.5% CO2; 37C Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative DNA Profile (STR): D5S818: 12; D13S317: 12, 14; D7S820: 10; D16S539: 11, 12; vWA: 14, 17; THO1: 6, 9.3; TPOX: 11, 12; CSF1PO: 10; Amelogenin: X
Male, Caucasian, 43 yrs of age Medium: RPMI-1640 Medium + 10% FBS Subculture: Cultures can be maintained by the addition of fresh medium. Seed cells at 8 X 104 to 3 x 10^5 cells/mL is recommended. Subculture when cell concentration is between 7 x 105 and 1 x 10^6 cells/mL; a certain amount of cell debris is always visible; A subcultivation ratio of 1:3 to 1:4 is recommended.5% CO2; 37C Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative DNA Profile (STR): D5S818: 12; D13S317: 12, 14; D7S820: 10; D16S539: 11, 12; vWA: 14, 17; THO1: 6, 9.3; TPOX: 11, 12; CSF1PO: 10; Amelogenin: X
RPMI-1640 Medium + 10% FBS Subculture: Cultures can be maintained by the addition of fresh medium. Seed cells at 8 X 104 to 3 x 10^5 cells/mL is recommended. Subculture when cell concentration is between 7 x 105 and 1 x 10^6 cells/mL; a certain amount of cell debris is always visible; A subcultivation ratio of 1:3 to 1:4 is recommended.5% CO2; 37C Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative DNA Profile (STR): D5S818: 12; D13S317: 12, 14; D7S820: 10; D16S539: 11, 12; vWA: 14, 17; THO1: 6, 9.3; TPOX: 11, 12; CSF1PO: 10; Amelogenin: X
Cultures can be maintained by the addition of fresh medium. Seed cells at 8 X 104 to 3 x 10^5 cells/mL is recommended. Subculture when cell concentration is between 7 x 105 and 1 x 10^6 cells/mL; a certain amount of cell debris is always visible; A subcultivation ratio of 1:3 to 1:4 is recommended.5% CO2; 37C Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative DNA Profile (STR): D5S818: 12; D13S317: 12, 14; D7S820: 10; D16S539: 11, 12; vWA: 14, 17; THO1: 6, 9.3; TPOX: 11, 12; CSF1PO: 10; Amelogenin: X
Complete culture medium supplemented with 5% (v/v) DMSO
I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative DNA Profile (STR): D5S818: 12; D13S317: 12, 14; D7S820: 10; D16S539: 11, 12; vWA: 14, 17; THO1: 6, 9.3; TPOX: 11, 12; CSF1PO: 10; Amelogenin: X
Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative DNA Profile (STR): D5S818: 12; D13S317: 12, 14; D7S820: 10; D16S539: 11, 12; vWA: 14, 17; THO1: 6, 9.3; TPOX: 11, 12; CSF1PO: 10; Amelogenin: X
Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative DNA Profile (STR): D5S818: 12; D13S317: 12, 14; D7S820: 10; D16S539: 11, 12; vWA: 14, 17; THO1: 6, 9.3; TPOX: 11, 12; CSF1PO: 10; Amelogenin: X
Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative DNA Profile (STR): D5S818: 12; D13S317: 12, 14; D7S820: 10; D16S539: 11, 12; vWA: 14, 17; THO1: 6, 9.3; TPOX: 11, 12; CSF1PO: 10; Amelogenin: X
HIV: Negative Hepatitis B: Negative Hepatitis C: Negative DNA Profile (STR): D5S818: 12; D13S317: 12, 14; D7S820: 10; D16S539: 11, 12; vWA: 14, 17; THO1: 6, 9.3; TPOX: 11, 12; CSF1PO: 10; Amelogenin: X
Hepatitis B: Negative Hepatitis C: Negative DNA Profile (STR): D5S818: 12; D13S317: 12, 14; D7S820: 10; D16S539: 11, 12; vWA: 14, 17; THO1: 6, 9.3; TPOX: 11, 12; CSF1PO: 10; Amelogenin: X
Hepatitis C: Negative DNA Profile (STR): D5S818: 12; D13S317: 12, 14; D7S820: 10; D16S539: 11, 12; vWA: 14, 17; THO1: 6, 9.3; TPOX: 11, 12; CSF1PO: 10; Amelogenin: X
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