NAMALWA cells

Disease:
Burkitt's lymphoma
Origin:
Established from the tumor mass of an African child with Burkitt lymphoma in 1967; cells express MYC mRNA
Species:
Homo sapiens
Tissue:
B lymphocyte
Morphology:
Lymphoblast; lymphoblastoid
Properties:
Single cells or small clusters in suspension
Cytogenic data:
Human hypodiploid karyotype with 4% polyploidy - 44(43-46)< 2n> X, -Y, -5, -6, +7, -10, -13, -21, +3mar, ins(1; ?)(p11; ?), ins(3)(q11q29), der(5)t(1; 5)(q25; q35), del(8)(p22), der(14)t(14; ?)(p11; ?), der(15)t(15; 21)(p11; q11) - a supernumerary ring or fragment present in 8% of cells - NAMALWA marker chromosomes present
Patient:
Female, African, 3 yrs of age
Medium:
RPMI-1640 Medium + 10% FBS + 10 mM HEPES + 1.0 mM sodium pyruvate
Subculture:
Cultures can be maintained by the addition of fresh medium or replacement of medium. Maintain at 0.5-1.5 x 10^6 cells/ml; split 1:2 to 1:3 every 2-3 days; subculture cells at 1 x 10^6 cells/ml; 5% CO2; 37C
Freezing Medium:
Complete culture medium supplemented with 5% (v/v) DMSO
Biosafety Level:
II [ Cells Contain Herpesvirus (EBV) and Squirrel monkey retrovirus (SMRV)]
Sterility:
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
Pathogens:
HIV: Negative
Hepatitis B: Negative
Hepatitis C: Negative
DNA Profile (STR): Amelogenin: X; CSF1PO: 10, 11; D13S317: 11, 12; D16S539: 9; D5S818: 12, 13; D7S820: 11; THO1: 7, 9.3; TPOX: 6, 11; vWA: 14

Cell Lines