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Osteosarcoma; multipotential sarcoma Origin: Derived from primary tumor of a patient diagnosed with primitive multipotential sarcoma of the femur Species:Homo sapiens Tissue:Bone Morphology:Fibroblast Properties:Adherent Cytogenic data:SJSA-1 cells also exhibit a 15 fold amplification of the gli proto-oncogene Patient:Male, Black, 19 years of age Medium:AddexBio-formulated RPMI-1640 Medium(C0004-01)+ 10% FBS Subculture:1:5 to 1:8 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37 degree C Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: SJSA-1 (AddexBio C0004004) Mutations in SJSA-1 Cells
Derived from primary tumor of a patient diagnosed with primitive multipotential sarcoma of the femur Species:Homo sapiens Tissue:Bone Morphology:Fibroblast Properties:Adherent Cytogenic data:SJSA-1 cells also exhibit a 15 fold amplification of the gli proto-oncogene Patient:Male, Black, 19 years of age Medium:AddexBio-formulated RPMI-1640 Medium(C0004-01)+ 10% FBS Subculture:1:5 to 1:8 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37 degree C Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: SJSA-1 (AddexBio C0004004) Mutations in SJSA-1 Cells
Homo sapiens Tissue:Bone Morphology:Fibroblast Properties:Adherent Cytogenic data:SJSA-1 cells also exhibit a 15 fold amplification of the gli proto-oncogene Patient:Male, Black, 19 years of age Medium:AddexBio-formulated RPMI-1640 Medium(C0004-01)+ 10% FBS Subculture:1:5 to 1:8 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37 degree C Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: SJSA-1 (AddexBio C0004004) Mutations in SJSA-1 Cells
Bone Morphology:Fibroblast Properties:Adherent Cytogenic data:SJSA-1 cells also exhibit a 15 fold amplification of the gli proto-oncogene Patient:Male, Black, 19 years of age Medium:AddexBio-formulated RPMI-1640 Medium(C0004-01)+ 10% FBS Subculture:1:5 to 1:8 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37 degree C Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: SJSA-1 (AddexBio C0004004) Mutations in SJSA-1 Cells
Fibroblast Properties:Adherent Cytogenic data:SJSA-1 cells also exhibit a 15 fold amplification of the gli proto-oncogene Patient:Male, Black, 19 years of age Medium:AddexBio-formulated RPMI-1640 Medium(C0004-01)+ 10% FBS Subculture:1:5 to 1:8 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37 degree C Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: SJSA-1 (AddexBio C0004004) Mutations in SJSA-1 Cells
Adherent Cytogenic data:SJSA-1 cells also exhibit a 15 fold amplification of the gli proto-oncogene Patient:Male, Black, 19 years of age Medium:AddexBio-formulated RPMI-1640 Medium(C0004-01)+ 10% FBS Subculture:1:5 to 1:8 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37 degree C Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: SJSA-1 (AddexBio C0004004) Mutations in SJSA-1 Cells
SJSA-1 cells also exhibit a 15 fold amplification of the gli proto-oncogene Patient:Male, Black, 19 years of age Medium:AddexBio-formulated RPMI-1640 Medium(C0004-01)+ 10% FBS Subculture:1:5 to 1:8 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37 degree C Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: SJSA-1 (AddexBio C0004004) Mutations in SJSA-1 Cells
Male, Black, 19 years of age Medium:AddexBio-formulated RPMI-1640 Medium(C0004-01)+ 10% FBS Subculture:1:5 to 1:8 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37 degree C Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: SJSA-1 (AddexBio C0004004) Mutations in SJSA-1 Cells
AddexBio-formulated RPMI-1640 Medium(C0004-01)+ 10% FBS Subculture:1:5 to 1:8 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37 degree C Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: SJSA-1 (AddexBio C0004004) Mutations in SJSA-1 Cells
1:5 to 1:8 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37 degree C Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: SJSA-1 (AddexBio C0004004) Mutations in SJSA-1 Cells
Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: SJSA-1 (AddexBio C0004004) Mutations in SJSA-1 Cells
I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: SJSA-1 (AddexBio C0004004) Mutations in SJSA-1 Cells
Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: SJSA-1 (AddexBio C0004004) Mutations in SJSA-1 Cells
Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: SJSA-1 (AddexBio C0004004) Mutations in SJSA-1 Cells
Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: SJSA-1 (AddexBio C0004004) Mutations in SJSA-1 Cells
HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: SJSA-1 (AddexBio C0004004) Mutations in SJSA-1 Cells
Hepatitis B: Negative Hepatitis C: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: SJSA-1 (AddexBio C0004004) Mutations in SJSA-1 Cells
Hepatitis C: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: SJSA-1 (AddexBio C0004004) Mutations in SJSA-1 Cells
If use of this culture results in a scientific publication, it should be cited in the publication as: SJSA-1 (AddexBio C0004004) Mutations in SJSA-1 Cells
Mutations in SJSA-1 Cells
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