JIMT-1 cells

Disease:
Breast carcinoma
Origin:
Established from pleural effusions of a breast cancer patient (grade 3 invasive, T2N1M0)
Species:
Homo sapiens
Tissue:
Breast, mammary gland
Morphology:
Epithelial
Properties:
Adherent
Cytogenic data:
Human flat-moded highly rearranged hyperdiploid karyotype with 6% polyploidy - 46-56< 2n> XX, +1, +2, +2, +3, +5, +6, +7, +12, -13, -14, -15, -15, -16, -18, +17, +5mar, add(X)(p22.1), add(1)(q11), del(1)(q32.2), der(1)add(1)(p21)add(1)(q22), der(1)t(1; 6)(p11; q1?1)t(6; 5)(q25; ?p13), add(2)(q11)x2, t(2; 3)(q10; p10)add(2)(q25-31), add(3)(p11), der(4)t(?3; 4)(?q24; p11), add(5)(q15), der(5)add(5)(p15.3)del(5)(q22), der(5)t(5; 8)(p11; p11), add(6)(p11)add(6)(q23), der(7)t(1; 7)(p13; q21), der(7)t(7; ?12)(p11; p11), add(8)(p11), i(8q) add(9)(p11), der(9)add(9)(p11)add(9)(q23), add(11)(p13), der(11)dup(11)(p11p15)add(11)(p15), der(11)t(1; 11)(q24; p12), add(12)(q21), der(12; ?15)(p11; q11), add(17)(p11), add(17)(p13), der(17)add(17)(p11)add(17)(q21)
Patient:
Female, 62 yrs of age
Medium:
AddexBio-formulated DMEM (C0003-01) + 10% FBS
Subculture:
1:2 to 1:6 (80-90%); using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37C
Freezing Medium:
Complete culture medium supplemented with 5% (v/v) DMSO
Biosafety Level:
I
Sterility:
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
Pathogens:
HIV: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Protein Expression: cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin -
DNA Profile (STR):
Amelogenin: X, X
CSF1PO: 12, 13
D13S317: 8, 13
D16S539: 11, 11
D5S818: 12, 13, 14
D7S820: 12, 12
THO1: 7, 7
TPOX: 8, 8
vWA: 18, 18
Citation Format:
If use of this culture results in a scientific publication, it should be cited in the publication as: JIMT-1 (AddexBio C0006005) Mutations in JIMT-1 Cells
Related cells by Organ: 4T1
AU-565
BT-20
BT-474
BT-483
BT-549
JIMT-1
MCF-7
SK-BR-3
T47D
ZR-75-1

Cell Lines