MCF-10A

Disease:

Fibrocystic disease

Origin:

MCF 10A was derived from adherent cells in the population.

Species:

Homo sapiens, human

Tissue:

Mammary gland; breast

Morphology:

Epithelial

Properties:

Adherent

Cytogenic data:

The MCF 10A cell line is a non-tumorigenic epithelial cell line. The cells are positive for epithelial sialomucins, cytokeratins and milk fat globule antigen. They exhibit three dimensional growth in collagen, and form domes in confluent cultures. Thus far, the cells have shown no signs of terminal differentiation or senescence. The line is responsive to insulin, glucocorticoids, cholera enterotoxin, and epidermal growth factor (EGF).

Patient:

Female, Caucasian, 36 years of age.

Medium:

DMEM/F12 (1:1) + 5% Horse Serum + EGF (20 ng/mL) + Hydrocortisone (0.5 ug/mL) + Cholera Toxin (100 ng/mL) + Insulin (10ug/mL).

Subculture:

Remove medium, and rinse with 0.05% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. Culture at 5% CO2, 37C. A subcultivation ratio of 1:3 to 1:4 is recommended. Renew medium once every 2 to 3 days depending on cell density.

Freezing Medium:

70% growth medium supplemented with 10% (v/v) DMSO and 20% horse serum