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Medulallary carcinoma
This cell line was isolated from pleural effusions.
Homo sapiens, human Tissue: Mammary gland; breast/medulla Morphology: Epithelial Properties: Adherent Cytogenic data: modal number =52 to 54; range = 52 to 69. Most of the karyotypes had three normal X chromosomes. Normal chromosomes N1, N5, N13, N14, N15, N16, N19, and N22 were not found. Seventeen marker chromosomes were identified: t(10q14q) der(12)t(1; 12)(q21; p13), der(7)t(7; ?)(q22; ?), del(1)(q11), t(3qter> 3q21::10p15> 10qter), 7q+, del(5)(q13), 19q+, t(10q; 14q), der(4)t(1; 4)(q21; q25), 9q+, 13p+, 15p+ unknown, unknown, unknown, del(6)(q21). Patient: Black, female, 44 years of age. Medium: AddexBio-formulated Leibovitz's L-15 Medium (C0006-01) + 10% FBSSubculture: A subcultivation ratio of 1:2 to 1:3 is recommended using 0.25% trypsin or trypsin/EDTA. Culture at 100% Air, 37C. Freezing Medium:Complete growth culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative DNA Profile (STR): Amelogenin: X CSF1PO: 10 D13S317: 11 D16S539: 11 D5S818: 12 D7S820: 10, 11 THO1:7, 8 TPOX: 11, 9 vWA: 15 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: MDA-MB-157 (AddexBio C0006020)
Mammary gland; breast/medulla Morphology: Epithelial Properties: Adherent Cytogenic data: modal number =52 to 54; range = 52 to 69. Most of the karyotypes had three normal X chromosomes. Normal chromosomes N1, N5, N13, N14, N15, N16, N19, and N22 were not found. Seventeen marker chromosomes were identified: t(10q14q) der(12)t(1; 12)(q21; p13), der(7)t(7; ?)(q22; ?), del(1)(q11), t(3qter> 3q21::10p15> 10qter), 7q+, del(5)(q13), 19q+, t(10q; 14q), der(4)t(1; 4)(q21; q25), 9q+, 13p+, 15p+ unknown, unknown, unknown, del(6)(q21). Patient: Black, female, 44 years of age. Medium: AddexBio-formulated Leibovitz's L-15 Medium (C0006-01) + 10% FBSSubculture: A subcultivation ratio of 1:2 to 1:3 is recommended using 0.25% trypsin or trypsin/EDTA. Culture at 100% Air, 37C. Freezing Medium:Complete growth culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative DNA Profile (STR): Amelogenin: X CSF1PO: 10 D13S317: 11 D16S539: 11 D5S818: 12 D7S820: 10, 11 THO1:7, 8 TPOX: 11, 9 vWA: 15 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: MDA-MB-157 (AddexBio C0006020)
Epithelial Properties: Adherent Cytogenic data: modal number =52 to 54; range = 52 to 69. Most of the karyotypes had three normal X chromosomes. Normal chromosomes N1, N5, N13, N14, N15, N16, N19, and N22 were not found. Seventeen marker chromosomes were identified: t(10q14q) der(12)t(1; 12)(q21; p13), der(7)t(7; ?)(q22; ?), del(1)(q11), t(3qter> 3q21::10p15> 10qter), 7q+, del(5)(q13), 19q+, t(10q; 14q), der(4)t(1; 4)(q21; q25), 9q+, 13p+, 15p+ unknown, unknown, unknown, del(6)(q21). Patient: Black, female, 44 years of age. Medium: AddexBio-formulated Leibovitz's L-15 Medium (C0006-01) + 10% FBSSubculture: A subcultivation ratio of 1:2 to 1:3 is recommended using 0.25% trypsin or trypsin/EDTA. Culture at 100% Air, 37C. Freezing Medium:Complete growth culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative DNA Profile (STR): Amelogenin: X CSF1PO: 10 D13S317: 11 D16S539: 11 D5S818: 12 D7S820: 10, 11 THO1:7, 8 TPOX: 11, 9 vWA: 15 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: MDA-MB-157 (AddexBio C0006020)
Adherent Cytogenic data: modal number =52 to 54; range = 52 to 69. Most of the karyotypes had three normal X chromosomes. Normal chromosomes N1, N5, N13, N14, N15, N16, N19, and N22 were not found. Seventeen marker chromosomes were identified: t(10q14q) der(12)t(1; 12)(q21; p13), der(7)t(7; ?)(q22; ?), del(1)(q11), t(3qter> 3q21::10p15> 10qter), 7q+, del(5)(q13), 19q+, t(10q; 14q), der(4)t(1; 4)(q21; q25), 9q+, 13p+, 15p+ unknown, unknown, unknown, del(6)(q21). Patient: Black, female, 44 years of age. Medium: AddexBio-formulated Leibovitz's L-15 Medium (C0006-01) + 10% FBSSubculture: A subcultivation ratio of 1:2 to 1:3 is recommended using 0.25% trypsin or trypsin/EDTA. Culture at 100% Air, 37C. Freezing Medium:Complete growth culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative DNA Profile (STR): Amelogenin: X CSF1PO: 10 D13S317: 11 D16S539: 11 D5S818: 12 D7S820: 10, 11 THO1:7, 8 TPOX: 11, 9 vWA: 15 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: MDA-MB-157 (AddexBio C0006020)
modal number =52 to 54; range = 52 to 69. Most of the karyotypes had three normal X chromosomes. Normal chromosomes N1, N5, N13, N14, N15, N16, N19, and N22 were not found. Seventeen marker chromosomes were identified: t(10q14q) der(12)t(1; 12)(q21; p13), der(7)t(7; ?)(q22; ?), del(1)(q11), t(3qter> 3q21::10p15> 10qter), 7q+, del(5)(q13), 19q+, t(10q; 14q), der(4)t(1; 4)(q21; q25), 9q+, 13p+, 15p+ unknown, unknown, unknown, del(6)(q21). Patient: Black, female, 44 years of age. Medium: AddexBio-formulated Leibovitz's L-15 Medium (C0006-01) + 10% FBSSubculture: A subcultivation ratio of 1:2 to 1:3 is recommended using 0.25% trypsin or trypsin/EDTA. Culture at 100% Air, 37C. Freezing Medium:Complete growth culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative DNA Profile (STR): Amelogenin: X CSF1PO: 10 D13S317: 11 D16S539: 11 D5S818: 12 D7S820: 10, 11 THO1:7, 8 TPOX: 11, 9 vWA: 15 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: MDA-MB-157 (AddexBio C0006020)
Black, female, 44 years of age. Medium: AddexBio-formulated Leibovitz's L-15 Medium (C0006-01) + 10% FBSSubculture: A subcultivation ratio of 1:2 to 1:3 is recommended using 0.25% trypsin or trypsin/EDTA. Culture at 100% Air, 37C. Freezing Medium:Complete growth culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative DNA Profile (STR): Amelogenin: X CSF1PO: 10 D13S317: 11 D16S539: 11 D5S818: 12 D7S820: 10, 11 THO1:7, 8 TPOX: 11, 9 vWA: 15 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: MDA-MB-157 (AddexBio C0006020)
AddexBio-formulated Leibovitz's L-15 Medium (C0006-01) + 10% FBSSubculture: A subcultivation ratio of 1:2 to 1:3 is recommended using 0.25% trypsin or trypsin/EDTA. Culture at 100% Air, 37C. Freezing Medium:Complete growth culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative DNA Profile (STR): Amelogenin: X CSF1PO: 10 D13S317: 11 D16S539: 11 D5S818: 12 D7S820: 10, 11 THO1:7, 8 TPOX: 11, 9 vWA: 15 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: MDA-MB-157 (AddexBio C0006020)
A subcultivation ratio of 1:2 to 1:3 is recommended using 0.25% trypsin or trypsin/EDTA. Culture at 100% Air, 37C. Freezing Medium:Complete growth culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative DNA Profile (STR): Amelogenin: X CSF1PO: 10 D13S317: 11 D16S539: 11 D5S818: 12 D7S820: 10, 11 THO1:7, 8 TPOX: 11, 9 vWA: 15 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: MDA-MB-157 (AddexBio C0006020)
Complete growth culture medium supplemented with 5% (v/v) DMSO
I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative DNA Profile (STR): Amelogenin: X CSF1PO: 10 D13S317: 11 D16S539: 11 D5S818: 12 D7S820: 10, 11 THO1:7, 8 TPOX: 11, 9 vWA: 15 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: MDA-MB-157 (AddexBio C0006020)
Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative DNA Profile (STR): Amelogenin: X CSF1PO: 10 D13S317: 11 D16S539: 11 D5S818: 12 D7S820: 10, 11 THO1:7, 8 TPOX: 11, 9 vWA: 15 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: MDA-MB-157 (AddexBio C0006020)
Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative DNA Profile (STR): Amelogenin: X CSF1PO: 10 D13S317: 11 D16S539: 11 D5S818: 12 D7S820: 10, 11 THO1:7, 8 TPOX: 11, 9 vWA: 15 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: MDA-MB-157 (AddexBio C0006020)
Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative DNA Profile (STR): Amelogenin: X CSF1PO: 10 D13S317: 11 D16S539: 11 D5S818: 12 D7S820: 10, 11 THO1:7, 8 TPOX: 11, 9 vWA: 15 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: MDA-MB-157 (AddexBio C0006020)
HIV: Negative Hepatitis B: Negative DNA Profile (STR): Amelogenin: X CSF1PO: 10 D13S317: 11 D16S539: 11 D5S818: 12 D7S820: 10, 11 THO1:7, 8 TPOX: 11, 9 vWA: 15 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: MDA-MB-157 (AddexBio C0006020)
Hepatitis B: Negative DNA Profile (STR): Amelogenin: X CSF1PO: 10 D13S317: 11 D16S539: 11 D5S818: 12 D7S820: 10, 11 THO1:7, 8 TPOX: 11, 9 vWA: 15 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: MDA-MB-157 (AddexBio C0006020)
Amelogenin: X CSF1PO: 10 D13S317: 11 D16S539: 11 D5S818: 12 D7S820: 10, 11 THO1:7, 8 TPOX: 11, 9 vWA: 15 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: MDA-MB-157 (AddexBio C0006020)
CSF1PO: 10 D13S317: 11 D16S539: 11 D5S818: 12 D7S820: 10, 11 THO1:7, 8 TPOX: 11, 9 vWA: 15 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: MDA-MB-157 (AddexBio C0006020)
D13S317: 11 D16S539: 11 D5S818: 12 D7S820: 10, 11 THO1:7, 8 TPOX: 11, 9 vWA: 15 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: MDA-MB-157 (AddexBio C0006020)
D16S539: 11 D5S818: 12 D7S820: 10, 11 THO1:7, 8 TPOX: 11, 9 vWA: 15 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: MDA-MB-157 (AddexBio C0006020)
D5S818: 12 D7S820: 10, 11 THO1:7, 8 TPOX: 11, 9 vWA: 15 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: MDA-MB-157 (AddexBio C0006020)
D7S820: 10, 11 THO1:7, 8 TPOX: 11, 9 vWA: 15 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: MDA-MB-157 (AddexBio C0006020)
THO1:7, 8 TPOX: 11, 9 vWA: 15 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: MDA-MB-157 (AddexBio C0006020)
TPOX: 11, 9 vWA: 15 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: MDA-MB-157 (AddexBio C0006020)
vWA: 15 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: MDA-MB-157 (AddexBio C0006020)
If use of this culture results in a scientific publication, it should be cited in the publication as: MDA-MB-157 (AddexBio C0006020)
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