HT-29 cells

Disease:
Colorectal adenocarcinoma
Origin:
Species:
Homo sapiens
Tissue:
Colon
Morphology:
Epithelial
Properties:
Adherent
Cytogenic data:
Tumorigenic, The stemline chromosome number is hypertriploid
Patient:
Female, Caucasian, 44 yrs of age
Medium:
McCoy's 5a (Gibco 16600082) + 10% FBS
Subculture:
1:3 to 1:8, (80-90%), 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C
Freezing Medium:
Complete culture medium supplemented with 5% (v/v) DMSO
Biosafety Level: I
Sterility:
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
Pathogens:
HIV: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Protein Expression: HLA A1, A3, B12, B17, Cw5, CD4 -, cell surface expression of galactose ceramide, Urokinase receptor(u-PAR), vitamin D (moderate expression)
Isoenzymes: Me-2, 1; PGM3, 1-2; PGM1, 1-2; ES-D, 1; AK-1, 1; GLO-1, 1-2; G6PD, B
DNA Profile (STR): Amelogenin: X, X; CSF1PO: 11, 12; D13S317: 11, 11; D16S539: 11, 12; D5S818: 11, 12; D7S820: 10; THO1: 6, 9; TPOX: 8, 9; vWA: 17, 19; D3S1358: 15, 17; D21S11: 29, 30; D18S51: 13; Penta E: 14, 16; Penta D: 11, 13; D8S1179: 10; FGA: 20, 22 Mutations in HT-29 Cells
Citation Format:
If use of this culture results in a scientific publication, it should be cited in the publication as: HT-29 (AddexBio C0009004)
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Cell Lines