DLD-1 cells

Disease:
Colorectal adenocarcinoma; Dukes' type C
Origin:
Carcinoembryonic antigen (CEA) 0.5 ng/10 exp6 cells/10 days; colon antigen 3; keratin
Species:
Homo sapiens
Tissue:
Colon
Morphology:
Epithelial
Properties:
Adherent
Cytogenic data:
This is a pseudodiploid human cell line
Patient:
Male, adult
Medium:
AddexBio-formulated RPMI-1640 Medium(C0004-01) + 10% FBS
Subculture:
1:3 to 1:10 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37C
Freezing Medium:
Complete culture medium supplemented with 5% (v/v) DMSO
Biosafety Level:
I
Sterility:
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
Pathogens:
HIV: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Protein Expression: cytokeratin +, cytokeratin-7 -, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, desmin -, endothel -, EpCAM -, GFAP -, neurofilament -, vimentin -
Isoenzymes: ES-D, 1-2; G6PD, B; PEP-D, 1; PGD, A; PGM1, 1; PGM3, 1
DNA Profile (STR): Amelogenin: X, Y; CSF1PO: 11, 12; D13S317: 8, 11; D16S539: 12, 13; D5S818: 13; D7S820: 10, 12; THO1: 7, 9.3; TPOX: 8, 11; vWA: 18, 19
Citation Format:
If use of this culture results in a scientific publication, it should be cited in the publication as: DLD-1 (AddexBio C0009007)
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