SNU-407 cells

Disease:
Colorectal adenocarcinoma
Origin:
Well-differentiated tumor cells derived from metastatic site: buttock.
Species:
Homo sapiens
Tissue:
Colon
Morphology:
Epithelial; monolayer.
Properties:
Adherent
Cytogenic data:
Tumorigenic.
Patient:
Male, Asian (Mongoloid), 37 yrs of age
Medium:
AddexBio-formulated RPMI-1640 Medium (C0004-01) + 10% FBS
Subculture:
1:3 to 1:4 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37 C
Freezing Medium:
Complete culture medium supplemented with 5% (v/v) DMSO
Biosafety Level:
I
Sterility:
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
Pathogens:
HIV: Negative
Hepatitis B: Negative
Hepatitis C: Negative
DNA Profile (STR):
Amelogenin: X, Y
CSF1PO: 10, 11
D13S317: 8, 10
D16S539: 11, 12
D5S818:10, 11
D7S820: 8, 10
THO1:6
TPOX: 7, 8
vWA: 14, 16
Mutations:
Microsatellite instability: Instable (MSI-high)
Doubling time: 42 hours
KRAS p.Gly12Asp (c.35G> A)
Kras2, exon 1;
hMSH2, exon 5;
beta-catenin, exon 5 Mutations in SNU-407 Cells
Citation Format:
If use of this culture results in a scientific publication, it should be cited in the publication as: SNU-407 (AddexBio C0009016)
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Cell Lines