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Hepatocellular carcinoma Origin: Established from the tumor tissue of an 8-year-old black boy Species:Homo sapiens Tissue:Liver Morphology:Epithelial Properties:Adherent Cytogenic data:Human hypotriploid stemline with near tetraploid side line Patient:Male, 8 years of age Medium:AddexBio-formulated EMEM (C0005-01) + 10% FBS Subculture:1:4 to 1:10 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37C Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: II Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Protein Expression: Alpha-foetoproteins, albumin, transferrin, fibrinogen, complement (C3), alpha-2 macroglobulins DNA Profile (STR): Amelogenin: X; CSF1PO: 8; D13S317: 12, 14; D16S539: 10; D5S818: 13; D7S820: 8, 10; THO1: 6, 7; TPOX: 9; vWA: 17 Related cells by Organ: Hep3B cells HepG2 cells
Established from the tumor tissue of an 8-year-old black boy Species:Homo sapiens Tissue:Liver Morphology:Epithelial Properties:Adherent Cytogenic data:Human hypotriploid stemline with near tetraploid side line Patient:Male, 8 years of age Medium:AddexBio-formulated EMEM (C0005-01) + 10% FBS Subculture:1:4 to 1:10 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37C Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: II Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Protein Expression: Alpha-foetoproteins, albumin, transferrin, fibrinogen, complement (C3), alpha-2 macroglobulins DNA Profile (STR): Amelogenin: X; CSF1PO: 8; D13S317: 12, 14; D16S539: 10; D5S818: 13; D7S820: 8, 10; THO1: 6, 7; TPOX: 9; vWA: 17 Related cells by Organ: Hep3B cells HepG2 cells
Homo sapiens Tissue:Liver Morphology:Epithelial Properties:Adherent Cytogenic data:Human hypotriploid stemline with near tetraploid side line Patient:Male, 8 years of age Medium:AddexBio-formulated EMEM (C0005-01) + 10% FBS Subculture:1:4 to 1:10 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37C Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: II Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Protein Expression: Alpha-foetoproteins, albumin, transferrin, fibrinogen, complement (C3), alpha-2 macroglobulins DNA Profile (STR): Amelogenin: X; CSF1PO: 8; D13S317: 12, 14; D16S539: 10; D5S818: 13; D7S820: 8, 10; THO1: 6, 7; TPOX: 9; vWA: 17 Related cells by Organ: Hep3B cells HepG2 cells
Liver Morphology:Epithelial Properties:Adherent Cytogenic data:Human hypotriploid stemline with near tetraploid side line Patient:Male, 8 years of age Medium:AddexBio-formulated EMEM (C0005-01) + 10% FBS Subculture:1:4 to 1:10 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37C Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: II Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Protein Expression: Alpha-foetoproteins, albumin, transferrin, fibrinogen, complement (C3), alpha-2 macroglobulins DNA Profile (STR): Amelogenin: X; CSF1PO: 8; D13S317: 12, 14; D16S539: 10; D5S818: 13; D7S820: 8, 10; THO1: 6, 7; TPOX: 9; vWA: 17 Related cells by Organ: Hep3B cells HepG2 cells
Epithelial Properties:Adherent Cytogenic data:Human hypotriploid stemline with near tetraploid side line Patient:Male, 8 years of age Medium:AddexBio-formulated EMEM (C0005-01) + 10% FBS Subculture:1:4 to 1:10 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37C Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: II Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Protein Expression: Alpha-foetoproteins, albumin, transferrin, fibrinogen, complement (C3), alpha-2 macroglobulins DNA Profile (STR): Amelogenin: X; CSF1PO: 8; D13S317: 12, 14; D16S539: 10; D5S818: 13; D7S820: 8, 10; THO1: 6, 7; TPOX: 9; vWA: 17 Related cells by Organ: Hep3B cells HepG2 cells
Adherent Cytogenic data:Human hypotriploid stemline with near tetraploid side line Patient:Male, 8 years of age Medium:AddexBio-formulated EMEM (C0005-01) + 10% FBS Subculture:1:4 to 1:10 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37C Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: II Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Protein Expression: Alpha-foetoproteins, albumin, transferrin, fibrinogen, complement (C3), alpha-2 macroglobulins DNA Profile (STR): Amelogenin: X; CSF1PO: 8; D13S317: 12, 14; D16S539: 10; D5S818: 13; D7S820: 8, 10; THO1: 6, 7; TPOX: 9; vWA: 17 Related cells by Organ: Hep3B cells HepG2 cells
Human hypotriploid stemline with near tetraploid side line Patient:Male, 8 years of age Medium:AddexBio-formulated EMEM (C0005-01) + 10% FBS Subculture:1:4 to 1:10 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37C Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: II Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Protein Expression: Alpha-foetoproteins, albumin, transferrin, fibrinogen, complement (C3), alpha-2 macroglobulins DNA Profile (STR): Amelogenin: X; CSF1PO: 8; D13S317: 12, 14; D16S539: 10; D5S818: 13; D7S820: 8, 10; THO1: 6, 7; TPOX: 9; vWA: 17 Related cells by Organ: Hep3B cells HepG2 cells
Male, 8 years of age Medium:AddexBio-formulated EMEM (C0005-01) + 10% FBS Subculture:1:4 to 1:10 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37C Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: II Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Protein Expression: Alpha-foetoproteins, albumin, transferrin, fibrinogen, complement (C3), alpha-2 macroglobulins DNA Profile (STR): Amelogenin: X; CSF1PO: 8; D13S317: 12, 14; D16S539: 10; D5S818: 13; D7S820: 8, 10; THO1: 6, 7; TPOX: 9; vWA: 17 Related cells by Organ: Hep3B cells HepG2 cells
AddexBio-formulated EMEM (C0005-01) + 10% FBS Subculture:1:4 to 1:10 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37C Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: II Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Protein Expression: Alpha-foetoproteins, albumin, transferrin, fibrinogen, complement (C3), alpha-2 macroglobulins DNA Profile (STR): Amelogenin: X; CSF1PO: 8; D13S317: 12, 14; D16S539: 10; D5S818: 13; D7S820: 8, 10; THO1: 6, 7; TPOX: 9; vWA: 17 Related cells by Organ: Hep3B cells HepG2 cells
1:4 to 1:10 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37C Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: II Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Protein Expression: Alpha-foetoproteins, albumin, transferrin, fibrinogen, complement (C3), alpha-2 macroglobulins DNA Profile (STR): Amelogenin: X; CSF1PO: 8; D13S317: 12, 14; D16S539: 10; D5S818: 13; D7S820: 8, 10; THO1: 6, 7; TPOX: 9; vWA: 17 Related cells by Organ: Hep3B cells HepG2 cells
Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: II Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Protein Expression: Alpha-foetoproteins, albumin, transferrin, fibrinogen, complement (C3), alpha-2 macroglobulins DNA Profile (STR): Amelogenin: X; CSF1PO: 8; D13S317: 12, 14; D16S539: 10; D5S818: 13; D7S820: 8, 10; THO1: 6, 7; TPOX: 9; vWA: 17 Related cells by Organ: Hep3B cells HepG2 cells
Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Protein Expression: Alpha-foetoproteins, albumin, transferrin, fibrinogen, complement (C3), alpha-2 macroglobulins DNA Profile (STR): Amelogenin: X; CSF1PO: 8; D13S317: 12, 14; D16S539: 10; D5S818: 13; D7S820: 8, 10; THO1: 6, 7; TPOX: 9; vWA: 17 Related cells by Organ: Hep3B cells HepG2 cells
Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Protein Expression: Alpha-foetoproteins, albumin, transferrin, fibrinogen, complement (C3), alpha-2 macroglobulins DNA Profile (STR): Amelogenin: X; CSF1PO: 8; D13S317: 12, 14; D16S539: 10; D5S818: 13; D7S820: 8, 10; THO1: 6, 7; TPOX: 9; vWA: 17 Related cells by Organ: Hep3B cells HepG2 cells
Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Protein Expression: Alpha-foetoproteins, albumin, transferrin, fibrinogen, complement (C3), alpha-2 macroglobulins DNA Profile (STR): Amelogenin: X; CSF1PO: 8; D13S317: 12, 14; D16S539: 10; D5S818: 13; D7S820: 8, 10; THO1: 6, 7; TPOX: 9; vWA: 17 Related cells by Organ: Hep3B cells HepG2 cells
HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Protein Expression: Alpha-foetoproteins, albumin, transferrin, fibrinogen, complement (C3), alpha-2 macroglobulins DNA Profile (STR): Amelogenin: X; CSF1PO: 8; D13S317: 12, 14; D16S539: 10; D5S818: 13; D7S820: 8, 10; THO1: 6, 7; TPOX: 9; vWA: 17 Related cells by Organ: Hep3B cells HepG2 cells
Hepatitis B: Negative Hepatitis C: Negative Protein Expression: Alpha-foetoproteins, albumin, transferrin, fibrinogen, complement (C3), alpha-2 macroglobulins DNA Profile (STR): Amelogenin: X; CSF1PO: 8; D13S317: 12, 14; D16S539: 10; D5S818: 13; D7S820: 8, 10; THO1: 6, 7; TPOX: 9; vWA: 17 Related cells by Organ: Hep3B cells HepG2 cells
Hepatitis C: Negative Protein Expression: Alpha-foetoproteins, albumin, transferrin, fibrinogen, complement (C3), alpha-2 macroglobulins DNA Profile (STR): Amelogenin: X; CSF1PO: 8; D13S317: 12, 14; D16S539: 10; D5S818: 13; D7S820: 8, 10; THO1: 6, 7; TPOX: 9; vWA: 17 Related cells by Organ: Hep3B cells HepG2 cells
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