H2170 cells

Disease:

Squamous cell carcinoma

Origin:

The line was established in April 1989 from a tissue donor who was a non-smoker.

Species:

Homo sapiens, human

Tissue:

Lung

Morphology:

Epithelial-like

Properties:

Adherent

Patient:

Male, non-smoker.

Medium:

AddexBio-formulated RPMI-1640 Medium (C0004-01) + 10% FBS

Subculture:

Remove medium, and rinse with 0.25% trypsin, 0.03 mM EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. Culture at 5% CO2, 37C. A subcultivation ratio of 1:3 to 1:6 is recommended. Renew medium once every 2 to 3 days depending on cell density.

Freezing Medium:

Complete culture medium supplemented with 5% (v/v) DMSO