Disease:
Choriocarcinoma
Origin:
This cell line was derived from a malignant gestational choriocarcinoma of the fetal placenta. This clone has proven useful in transport studies because the cells form polarized, confluent monolayers.
Species:
Homo sapiens Tissue:
Placenta Morphology:
Epithelial Properties:
Adherent Cytogenic data:
Modal number = 86; range = 71 to 178. Stemline number is hypotetraploid. Karyotype relatively stable within stemline number. Patient:
Male Medium:
AddexBio-Optimized DMEM (C0003-02) + 10% FBS Subculture:
Subculture at 1:4; feed cells 3 to 4 times weekly. Culture at 5% CO2; 37C Freezing Medium:
Complete culture medium supplemented with 5% (v/v) DMSO
Biosafety Level:
I Sterility:
Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens:
HIV: Negative Hepatitis B: Negative Hepatitis C: Negative DNA Profile (STR):
Amelogenin: X, Y CSF1PO: 11, 12 D13S317: 9, 11 D16S539: 13, 14 D5S818: 10, 11 D7S820: 10, 12 THO1: 9, 9.3 TPOX: 8 vWA: 16