IMR-90

Disease:
Normal
Origin:
The human diploid fibroblast strain IMR-90 was derived by W.W. Nichols and associates from the lungs of a 16-week female fetus.
Species:
Homo sapiens, human
Tissue:
Lung
Morphology:
Fibroblast
Properties:
Adherent
Cytogenic data:
normal human female; diploid; stable. Cytogenetic instability has been reported in the literature for some cell lines.
Patient:
Female, Caucasian, 16 weeks gestation.
Medium:
AddexBio-formulated EMEM (C0005-01) + 10% FBS
Subculture:
Remove medium, and rinse with 0.05% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. Culture at 5% CO2, 37C. A subcultivation ratio of 1:2 to 1:8 is recommended. Renew medium once every 2 to 3 days depending on cell density.
Freezing Medium:
Complete growth medium supplemented with 5% (v/v) DMSO
Biosafety Level:
I
Sterility:
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
Pathogens:
HIV: Negative
Hepatitis B: Negative
DNA Profile (STR):
Amelogenin: X
CSF1PO: 11, 13
D13S317: 11, 13
D16S539: 10, 13
D5S818:12, 13
D7S820:9, 12
THO1:8, 9.3
TPOX: 8, 9
vWA: 16, 19
Citation Format:
If use of this culture results in a scientific publication, it should be cited in the publication as: IMR-90 (AddexBio P0016019)

Cell Lines