IMR-90

Disease:

Normal

Origin:

The human diploid fibroblast strain IMR-90 was derived by W.W. Nichols and associates from the lungs of a 16-week female fetus.

Species:

Homo sapiens, human

Tissue:

Lung

Morphology:

Fibroblast

Properties:

Adherent

Cytogenic data:

normal human female; diploid; stable. Cytogenetic instability has been reported in the literature for some cell lines.

Patient:

Female, Caucasian, 16 weeks gestation.

Medium:

AddexBio-formulated EMEM (C0005-01) + 10% FBS

Subculture:

Remove medium, and rinse with 0.05% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. Culture at 5% CO2, 37C. A subcultivation ratio of 1:2 to 1:8 is recommended. Renew medium once every 2 to 3 days depending on cell density.

Freezing Medium:

Complete growth medium supplemented with 5% (v/v) DMSO