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Immortalized pancreatic ductal epithelial cells. Species:Homo sapiens Tissue:Pancreas Morphology:Epithelial Properties:Adherent Cytogenic data:Normal, pancretic duct epithelial cells immortalized with HPV16 E6 and E7, normal genotype and phenotype (near normal), and Non-tumorigenic Medium:Keratinocyte SFM + Supplements Subculture:1:2 to 1:3 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37 C Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: II Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative
Homo sapiens Tissue:Pancreas Morphology:Epithelial Properties:Adherent Cytogenic data:Normal, pancretic duct epithelial cells immortalized with HPV16 E6 and E7, normal genotype and phenotype (near normal), and Non-tumorigenic Medium:Keratinocyte SFM + Supplements Subculture:1:2 to 1:3 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37 C Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: II Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative
Pancreas Morphology:Epithelial Properties:Adherent Cytogenic data:Normal, pancretic duct epithelial cells immortalized with HPV16 E6 and E7, normal genotype and phenotype (near normal), and Non-tumorigenic Medium:Keratinocyte SFM + Supplements Subculture:1:2 to 1:3 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37 C Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: II Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative
Epithelial Properties:Adherent Cytogenic data:Normal, pancretic duct epithelial cells immortalized with HPV16 E6 and E7, normal genotype and phenotype (near normal), and Non-tumorigenic Medium:Keratinocyte SFM + Supplements Subculture:1:2 to 1:3 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37 C Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: II Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative
Adherent Cytogenic data:Normal, pancretic duct epithelial cells immortalized with HPV16 E6 and E7, normal genotype and phenotype (near normal), and Non-tumorigenic Medium:Keratinocyte SFM + Supplements Subculture:1:2 to 1:3 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37 C Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: II Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative
Normal, pancretic duct epithelial cells immortalized with HPV16 E6 and E7, normal genotype and phenotype (near normal), and Non-tumorigenic Medium:Keratinocyte SFM + Supplements Subculture:1:2 to 1:3 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37 C Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: II Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative
Keratinocyte SFM + Supplements Subculture:1:2 to 1:3 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37 C Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: II Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative
1:2 to 1:3 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37 C Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: II Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative
Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: II Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative
II Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative
Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative
Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative
Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative
HIV: Negative Hepatitis B: Negative Hepatitis C: Negative
Hepatitis B: Negative Hepatitis C: Negative
Hepatitis C: Negative
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