VERO cells

Disease:

Normal

Origin:

The Vero cell line was initiated from the kidney of a normal adult African green monkey

Species:

African Green Monkey (Cercopithecus aethiops)

Tissue:

Kidney

Morphology:

Epithelial

Properties:

Adherent

Cytogenic data:

This is a cell line with the hypodiploid chromosome count. The modal chromosome number was 58 occurring in 66% of cells. In most cells, over 50% of the chromosomes in each cell complement belonged to structurally altered marker chromosomes. Normal A3, A4, B4, and B5 were absent; B2, B3 and B7 were occasionally paired; and B9, C1 and C5 were mostly paired. The rate of cells with higher ploidies was 1.7%. Other chromosomes were mostly present in single copy.

Medium:

MEM Medium + 10% FBS

Subculture:

When confluent using 0.25% trypsin or trypsin/EDTA, subculture at 1:3 to 1:6; feed cells 2 to 3 times weekly. Culture at 5% CO2, 37C.

Freezing Medium:

Complete culture medium supplemented with 5% (v/v) DMSO