FO cells

Disease:
Plasmacytoma; myeloma
Origin:
The line was made by self fusing Sp2/0 myeloma cells using Sendai virus.
Species:
Mus musculus, mouse
Tissue:
B lymphoblast
Morphology:
Lymphoblast
Properties:
Suspension, tendency to stick on the flask
Uses:
The line can be used as a fusion partner for making B cell hybridomas. The cells are resistant to 8-azaguanine and do not produce immunoglobulin.
Patient:
Mouse
Medium:
DMEM Medium + 10% FBS
Subculture:
Cultures can be maintained by adding fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 0.5 to 1 X 10^5 viable cells/mL. Change medium every 2-3 days. Maintain cell density below 10^6 viable cells/mL. Culture at 5% CO₂; 37C.
Freezing Medium:
Complete culture medium supplemented with 10% (v/v) DMSO
Biosafety Level:
I
Sterility:
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
Pathogens:
HIV: Negative
Hepatitis B: Negative
Hepatitis C: Negative

Cell Lines