NALM-6 cells

Disease:
Acute lymphoblastic leukemia (ALL)
Origin:
The line was established from the peripheral blood of a 19-year-old man with acute lymphoblastic leukemia (ALL) in relapse in 1976.
Species:
Homo sapiens, human
Tissue:
Peripheral blood
Morphology:
Lymphocyte-like
Properties:
Single, small, round cells in suspension
Cytogenic data:
human near diploid karyotype - 46(43-47)< 2n> XY, t(5; 12)(q33.2; p13.2)
Patient:
Male, 19 years of age.
Medium:
AddexBio-formulated RPMI-1640 Medium (C0004-01) + 10% FBS
Subculture:
Cultures can be maintained by addition or replacement of fresh medium. Start cultures between 2 X 10^5 cells/mL and 4 X 10^5cells/mL maintain between 3 X 10^5 and 3 X 10^6 cells/mL. Culture at 5% CO2, 37C.
Freezing Medium:
Complete growth culture medium supplemented with 7.5% (v/v) DMSO
Biosafety Level:
I
Sterility:
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
Pathogens:
HIV: Negative
Hepatitis B: Negative
DNA Profile (STR):
Amelogenin: X, Y
CSF1PO: 12
D13S317: 9, 11
D16S539: 11
D5S818:11
D7S820: 8, 12
THO1:8, 9
TPOX: 8, 10
vWA: 15, 18
Citation Format:
If use of this culture results in a scientific publication, it should be cited in the publication as: NALM-6 (AddexBio C0003030)

Cell Lines