A-172 cells

Disease:

Glioblastoma

Origin:

Derived from a glioblastoma removed from a 53 year old male. Non-tumourigenic in anti-thymocyte serum treated NIH Swiss mice.

Species:

Homo sapiens, human

Tissue:

Brain

Morphology:

Epithelial-like with processes and/or rounded

Properties:

Adherent

Cytogenic data:

n = 80, over 20 marker chromosomes per cell (hypertriploid)

Patient:

Male, 53 years of age

Medium:

AddexBio-formulated DMEM (C0003-01) + 10% FBS

Subculture:

Subculture sub-confluent cultures (70-80%) at 1:3 to 1:8 ratio, using 0.25% (w/v) Trypsin- 0.53 mM EDTA solution. Culture at 5% CO2, 37C.

Freezing Medium:

Complete growth culture medium supplemented with 7.5% (v/v) DMSO