bEnd.3 cells

Disease:

Endothelioma

Origin:

The cells were transformed by infection with the NTKmT retrovirus vector that expresses polyomavirus middle T antigen.

Species:

Mus musculus, mouse

Tissue:

Brain, cerebral cortex

Morphology:

Endothelial, endothelialpolyoma middle T antigen transformed

Properties:

Adherent

Strain:

BALB/c, 6 weeks

Medium:

AddexBio-formulated DMEM (C0003-01) + 10% FBS

Subculture:

Subculture sub-confluent cultures (70-80%) at 1:6 to 1:10 ratio, using 0.25% (w/v) Trypsin- 0.53 mM EDTA solution. Culture at 5% CO2, 37C.

Freezing Medium:

Complete growth culture medium supplemented with 7.5% (v/v) DMSO