bEnd.3 cells

Disease:
Endothelioma
Origin:
The cells were transformed by infection with the NTKmT retrovirus vector that expresses polyomavirus middle T antigen.
Species:
Mus musculus, mouse
Tissue:
Brain, cerebral cortex
Morphology:
Endothelial, endothelialpolyoma middle T antigen transformed
Properties:
Adherent
Strain:
BALB/c, 6 weeks
Medium:
AddexBio-formulated DMEM (C0003-01) + 10% FBS
Subculture:
Subculture sub-confluent cultures (70-80%) at 1:6 to 1:10 ratio, using 0.25% (w/v) Trypsin- 0.53 mM EDTA solution. Culture at 5% CO2, 37C.
Freezing Medium:
Complete growth culture medium supplemented with 7.5% (v/v) DMSO
Biosafety Level:
I
Sterility:
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
Pathogens:
HIV: Negative
Hepatitis B: Negative
Citation Format:
If use of this culture results in a scientific publication, it should be cited in the publication as: bEnd.3 (AddexBio C0005030)

Cell Lines