Hep-2C cells

Origin:
Cervix carcinoma
Origin:
Cells of this line contain HeLa marker chromosomes, and were derived via HeLa contamination. This line was originally thought to be derived from an epidermoid carcinoma of the larynx, but was subsequently found, based on isoenzyme analysis, HeLa marker chromosomes, and DNA fingerprinting, to have been established via HeLa cell contamination. The cells are positive for keratin by immunoperoxidase staining.
Species:
Homo sapiens, human
Tissue:
HeLa derivative, human cervix carcinoma
Morphology:
Epithelial
Properties:
Adherent
Cytogenic data:
Modal no. 76
Patient:
Female, Black
Medium:
AddexBio-formulated EMEM (C0005-01) + 10% FBS
Subculture:
Subculture sub-confluent cultures (85%) at 1:3 to 1:10 ratio, using 0.25% (w/v) Trypsin- 0.53 mM EDTA solution. Culture at 5% CO2, 37C.
Freezing Medium:
Complete growth culture medium supplemented with 7.5% (v/v) DMSO
Biosafety Level:
II
Sterility:
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
Pathogens:
HIV: Negative
Hepatitis B: Negative
HPV: Negative
EBV: Negative
CMV: Negative
DNA Profile (STR):
Amelogenin: X
CSF1PO: 9, 10
D13S317: 12, 13.3
D16S539: 9, 10
D5S818:11, 12
D7S820: 8, 12
THO1:7
TPOX: 8, 12
vWA: 16, 18
Citation Format:
If use of this culture results in a scientific publication, it should be cited in the publication as: Hep-2C (AddexBio C0008008)

Cell Lines