H1299 cells

Origin:
Carcinoma; non-small cell lung cancer
Origin:
The cell line was established from a lymph node metastasis of the lung from a patient who had received prior radiation therapy.
Species:
Homo sapiens, human
Tissue:
Lung; derived from metastatic site: lymph node
Morphology:
Epithelial
Properties:
Adherent
Patient:
Male, Caucasian, 43 years of age
Medium:
AddexBio-specialized RPMI-1640 Medium (C0004-01) + 10% FBS
Subculture:
Subculture sub-confluent cultures (85%) at 1:3 to 1:6 ratio, using 0.25% (w/v) Trypsin- 0.53 mM EDTA solution. Culture at 5% CO2, 37C.
Freezing Medium:
Complete growth culture medium supplemented with 7.5% (v/v) DMSO
Biosafety Level:
I
Sterility:
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
Pathogens:
HIV: Negative
Hepatitis B: Negative
HPV: Negative
EBV: Negative
CMV: Negative
DNA Profile (STR):
Amelogenin: X
CSF1PO: 12
D13S317: 12
D16S539: 12, 13
D5S818:11
D7S820: 10
THO1:6, 9.3
TPOX: 8
vWA: 16, 17, 18
Citation Format:
If use of this culture results in a scientific publication, it should be cited in the publication as: H1299 (AddexBio C0016053)

Cell Lines