H1299 cells

Origin:

Carcinoma; non-small cell lung cancer

Origin:

The cell line was established from a lymph node metastasis of the lung from a patient who had received prior radiation therapy.

Species:

Homo sapiens, human

Tissue:

Lung; derived from metastatic site: lymph node

Morphology:

Epithelial

Properties:

Adherent

Patient:

Male, Caucasian, 43 years of age

Medium:

AddexBio-specialized RPMI-1640 Medium (C0004-01) + 10% FBS

Subculture:

Subculture sub-confluent cultures (85%) at 1:3 to 1:6 ratio, using 0.25% (w/v) Trypsin- 0.53 mM EDTA solution. Culture at 5% CO2, 37C.

Freezing Medium:

Complete growth culture medium supplemented with 7.5% (v/v) DMSO