H446 cells

Origin:

Carcinoma; small cell lung cancer

Origin:

The H446 cell line was derived in 1982 from the pleural fluid of a patient with small cell cancer of the lung.

Species:

Homo sapiens, human

Tissue:

Lung; derived from metastatic site: pleural effusion

Morphology:

Epithelial

Properties:

Mixed, adherent and suspension

Cytogenic data:

This is a hypertriploid human cell line. The modal chromosome number is 74, occurring at 22% with polyploidy at 2.5%. Over 25 markers were common to most cells including inv(1)(p32q21), der(1)t(1:4)(q42; q11), i(5q), t(2p13q) and der(19)t(7; HSR; 19) (q11; HSR; p13.3). Structurally normal N1 and N13 were absent and normal N4, N5 and N10 were mostly single copies per cell. There were two X/Y pairs per cell.

Patient:

Male, Caucasian, 61 years of age

Medium:

AddexBio-formulated RPMI-1640 Medium(C0004-01) + 10% FBS

Subculture:

Subculture sub-confluent cultures (85%) at 1:3 to 1:9 ratio, using 0.25% (w/v) Trypsin- 0.53 mM EDTA solution. Culture at 5% CO2, 37C.

Freezing Medium:

Complete growth culture medium supplemented with 7.5% (v/v) DMSO