SKNBE2 [SK-N-BE(2)] cells

Origin:
Neuroblastoma
Origin:
The SK-N-BE(2) neuroblastoma cell line was established in November of 1972 from a bone marrow biopsy taken from child with disseminated neuroblastoma after repeated courses of chemotherapy and radiotherapy.
Species:
Homo sapiens, human
Tissue:
Brain; derived from metastatic site: bone marrow
Morphology:
Neuroblast, neuroblastoma
Properties:
Mixed, adherent and suspension
Cytogenic data:
Modal number = 44; one or more HSR bearing chromosomes
Patient:
Male, 2 years
Medium:
F12 Medium/AddexBio-formulated EMEM 1:1 mixture (C0005-01) + 10% FBS
Subculture:
Subculture sub-confluent cultures (85%) at 1:12 to 1:20 ratio, using 0.25% (w/v) Trypsin- 0.53 mM EDTA solution. Culture at 5% CO2, 37C.
Freezing Medium:
Complete growth culture medium supplemented with 7.5% (v/v) DMSO
Biosafety Level:
I
Sterility:
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
Pathogens:
HIV: Negative
Hepatitis B: Negative
HPV: Negative
EBV: Negative
CMV: Negative
DNA Profile (STR):
Amelogenin: X, Y
CSF1PO: 10
D13S317: 11
D16S539: 9, 11
D5S818:12
D7S820: 9, 10
THO1:6, 7
TPOX: 8, 11
vWA: 18
Citation Format:
If use of this culture results in a scientific publication, it should be cited in the publication as: SKNBE2 (AddexBio C0016055)

Cell Lines