SKNBE2 [SK-N-BE(2)] cells

Origin:

Neuroblastoma

Origin:

The SK-N-BE(2) neuroblastoma cell line was established in November of 1972 from a bone marrow biopsy taken from child with disseminated neuroblastoma after repeated courses of chemotherapy and radiotherapy.

Species:

Homo sapiens, human

Tissue:

Brain; derived from metastatic site: bone marrow

Morphology:

Neuroblast, neuroblastoma

Properties:

Mixed, adherent and suspension

Cytogenic data:

Modal number = 44; one or more HSR bearing chromosomes

Patient:

Male, 2 years

Medium:

F12 Medium/AddexBio-formulated EMEM 1:1 mixture (C0005-01) + 10% FBS

Subculture:

Subculture sub-confluent cultures (85%) at 1:12 to 1:20 ratio, using 0.25% (w/v) Trypsin- 0.53 mM EDTA solution. Culture at 5% CO2, 37C.

Freezing Medium:

Complete growth culture medium supplemented with 7.5% (v/v) DMSO