Alpha TC1-9 cells

Disease:

Mouse islet cell adenoma

Origin:

Alpha TC1 clone 9 is a pancreatic alpha cell line cloned from the alpha TC1 cell line which was derived from an adenoma created in transgenic mice expressing the SV40 large T antigen oncogene under the control of the rat preproglucagon promoter. This cell line is heterozygous for MHC alleles derived from both parental strains used in construction of the transgenic mice [C57BL/6J(H-2b] and DBA/2J(H-2d)].

Species:

Mus musculus, mouse

Tissue:

Pancreas; alpha cell

Morphology:

Epithelial-like

Properties:

Adherent; single cells and loosely attached clusters

Strain:

(C57BL/6J x DBA/2)F2 (transgenic for the SV40 T antigen)

Medium:

AddexBio-specialized DMEM (C0003-03) + 0.1 mM Non-essential amino acids (NEAA) + 10% FBS

Subculture:

Subculture sub-confluent cultures (70-80%) at 1:3 to 1:4 ratio, using 0.25% (w/v) Trypsin- 0.53 mM EDTA solution. Culture at 10% CO2, 37C.

Freezing Medium:

Complete growth culture medium supplemented with 7.5% (v/v) DMSO