AsPC-1 cells

Origin:
Adenocarcinoma
Origin:
The line was derived from nude mouse xenografts initiated with cells from the ascites of a patient with cancer of the pancreas.
Species:
Homo sapiens, human
Tissue:
Pancreas; derived from metastatic site: ascites
Morphology:
Epithelial-like and rounded
Properties:
Adherent
Patient:
Female, Caucasian, 62 years of age
Medium:
AddexBio-formulated RPMI-1640 Medium (C0004-01) + 10% FBS
Subculture:
Subculture sub-confluent cultures (85%) at 1:3 to 1:6 ratio, using 0.25% (w/v) Trypsin- 0.53 mM EDTA solution. Culture at 5% CO2, 37C.
Freezing Medium:
Complete growth culture medium supplemented with 7.5% (v/v) DMSO
Biosafety Level:
I
Sterility:
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
Pathogens:
HIV: Negative
Hepatitis B: Negative
HPV: Negative
EBV: Negative
CMV: Negative
DNA Profile (STR):
Amelogenin: X
CSF1PO: 10, 13
D13S317: 9, 12
D16S539: 11
D5S818:12
D7S820: 12, 13
THO1:7, 9.3
TPOX: 8, 10
vWA: 17
Citation Format:
If use of this culture results in a scientific publication, it should be cited in the publication as: AsPC-1 (AddexBio C0018023)

Cell Lines