AsPC-1 cells

Origin:

Adenocarcinoma

Origin:

The line was derived from nude mouse xenografts initiated with cells from the ascites of a patient with cancer of the pancreas.

Species:

Homo sapiens, human

Tissue:

Pancreas; derived from metastatic site: ascites

Morphology:

Epithelial-like and rounded

Properties:

Adherent

Patient:

Female, Caucasian, 62 years of age

Medium:

AddexBio-formulated RPMI-1640 Medium (C0004-01) + 10% FBS

Subculture:

Subculture sub-confluent cultures (85%) at 1:3 to 1:6 ratio, using 0.25% (w/v) Trypsin- 0.53 mM EDTA solution. Culture at 5% CO2, 37C.

Freezing Medium:

Complete growth culture medium supplemented with 7.5% (v/v) DMSO