L929 cells

Disease:

Normal adipose fibroblast

Origin:

The parent L strain was derived from normal subcutaneous areolar and adipose tissue of a 100-day-old male C3H/An mouse by WR Earle in 1940. NCTC clone 929 Clone of strain L (also known as L929) was subsequently derived in March 1948. Strain L was one of the first cell strains to be established in continuous culture, and clone 929 was the first cloned strain developed. Clone 929 was established (by the capillary technique for single cell isolation) from the 95th subculture generation of the parent strain. Cells are APRT+ and HPRT+.

Species:

Mus musculus, mouse

Tissue:

Subcutaneous connective tissue; areolar and adipose

Morphology:

Fibroblast

Properties:

Adherent

Cytogenic data:

modal chromosome number = 66; range = 65 to 68. There were approximately 20 to 30 marker chromosomes present in each metaphase spread. A high percentage of those markers were common to most analyzed cells. A long metacentric chromosome with secondary constriction was noted in 77/100 cells.

Strain:

Male, C3H/An, 100 days

Medium:

AddexBio-formulated EMEM (C0005-01) + 10% horse serum

Subculture:

Subculture sub-confluent cultures (85%) at 1:2 to 1:8 ratio, using 0.25% (w/v) Trypsin- 0.53 mM EDTA solution. Culture at 5% CO2, 37C.

Freezing Medium:

Complete growth culture medium supplemented with 7.5% (v/v) DMSO