BJ cells

Disease:

Normal

Origin:

The line was established from skin taken from normal foreskin.

Species:

Homo sapiens, human

Tissue:

Skin; foreskin

Morphology:

Fibroblast-like; fibroblast

Properties:

Adherent

Cytogenic data:

These cells have a reported normal diploid karyotype at population doubling 61 but an abnormal karyotype at population doubling 82. They are telomerase negative.

Patient:

Male, newborn.

Medium:

AddexBio-formulated EMEM (C0005-01) + 10% FBS

Subculture:

Remove medium, and rinse with 0.05% trypsin, 0.03 mM EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. Culture at 5% CO2, 37C. A subcultivation ratio of 1:2 to 1:9 is recommended. Renew medium once every 2 to 3 days depending on cell density.

Freezing Medium:

Complete culture medium supplemented with 5% (v/v) DMSO