CHO-hNCX1 cells

Channel/Receptor:
Sodium/calcium exchanger
Background :
The sodium-calcium exchanger (NCX) is an antiporter membrane protein that removes calcium from cells. It uses the energy that is stored in the electrochemical gradient of sodium (Na⁺) by allowing Na+ to flow down its gradient across the plasma membrane in exchange for the countertransport of calcium ions (Ca²⁺). The NCX removes a single calcium ion in exchange for the import of three sodium ions. The exchanger exists in many different cell types and animal species. The NCX is considered one of the most important cellular mechanisms for removing Ca²⁺.
Expression System :
CHO-K1 cells
Growth Condition :
Ham's F12 + 10% FBS + supplements (details enclosed in shipping Information Sheet)
Subculture :
1:2 to 1:3 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37C
Morphology and Properties :
Adherent epithelium
Gene Name :
NCX1, SLC8A1
Sequence :
GenBank accession number NM_001112802
Packaging :
Cryopreserved cells, 1 x 10⁶ cells/vial
Freezing Medium:
Complete culture medium supplemented with 5% (v/v) DMSO
Storage Recommendation :
Vapor phase of liquid nitrogen
Biosafety Level:
I
Sterility:
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
Pathogens:
HIV: Negative
Hepatitis B: Negative
Hepatitis C: Negative
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