R2C cells

Disease:
Leydig cell tumor
Origin:
Derived from a 2-month-old male WFu rat.
Species:
Rattus norvegicus, rat
Tissue:
Testis
Morphology:
Epithelial
Properties:
Adherent
Characteristics:
Virus susceptibility: herpes simplex; vaccinia; pseudorabies; vesicular stomatitis (Indiana). Resistant to poliovirus 2. Stimulation by other hormones or cAMP is not required for hormone secretion.
Patient:
Male, Rat, 2-month old
Medium:
Hams F12 Medium + 15% horse serum + 2.5% FBS
Subculture:
Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-5x10, 000 cells/cm² using 0.25% trypsin/EDTA; Culture at 5% CO2; 37C
Freezing Medium:
Complete culture medium supplemented with 10% (v/v) DMSO
Biosafety Level:
I
Sterility:
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
Pathogens:
HIV: Negative
Hepatitis B: Negative
Hepatitis C: Negative

Cell Lines