Active Recombinant Human Activin A protein

Recombinant human Activin A is a polypeptide chain containing 116 amino acids (311– 426 P08476 INHBA_HUMAN) and a His-tag at the N-terminal end. Several forms are observed with different predicted molecular masses such as monomer (13.7 kDa), dimmer ( 27.4 kDa) and multimeric forms.

7.27

P08476

Human recombinant protein expressed in Nicotiana benthamiana. It is produced by transient expression in non-transgenic plants. This product contains no animal–derived components or impurities. Animal Free product.

HHHHHHGLECDGKVNICCKKQFFVSFKDIGWNDWI IAPSGYHANYCEGECPSHIAGTSGSSLSFHSTVIN HYRMRGHSPFANLKSCCVPTKLRPMSMLYYDDGQN IIKKDIQNMIVEECGCS

Purity >97% by SDS-PAGE gel

1. Effect of rh Activin A on mouse plasmacytoma cell line (MPC-11) cells proliferation. - The biological activity of Activin A is measured by its ability to inhibit mouse plasmacytoma cell line (MPC-11) cells proliferation. Cell proliferation was measured by MTT method. ED50 <= 10ng/ml.

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- Vale W., Hseuh A., Rivier C. and Yu J. (1990). The inhibin/Activin family of hormones and growth factors. In Peptide Growth Factors and their Receptors: Handbook of Experimental Physiology, 95: 211–248. Eds M Sporn & A Roberts. Berlin: Springer-Verlag. - Schwall R. H., and Lai, C. (1991). Erythroid differentiation bioassys for activin. Methods Enzymol, 198: 340-346. - Sulyok S., Wankell M., Alzheimer C. and Werner S. (2004). Activin: an important regulator of wound repair, fibrosis, and neuroprotection. Mol. Cell. Endocrinology, 225 (1-2): 127–32. - Bamberger C., Schärer A., Antsiferova M., Tychsen B., Pankow S., Müller M., Rülicke T., Paus R. and Werner S. (2005). Activin controls skin morphogenesis and wound repair predominantly via stromal cells and in a concentration-dependent manner via keratinocytes. Am. J. Pathol., 167 (3): 733–47. - Chen Y. G., Wang Q., Lin S. L., Chang C. D., Chuang J., Chung J. and Ying S. Y. (2006). Activin signalling and its role in regulation of cell proliferation, apoptosis, and carcinogenesis. Exp. Biol. Med. (Maywood), 231 (5): 534–44. - Phillips D. J., Brauman J. N., Mason A. J., Kretser D.M and Hedger M. P.(1999). A sensitive and specific in vitro bioassay for activin using a mouse plasmacytoma cell line, MPC-11. J. of Endocrinology, 162: 111-116.

This lyophilized preparation is stable at 2-8C C for short term, long storage it should be kept at -20C. Reconstituted protein should be stored in working aliquots at –20C. Repeated freezing and thawing is not recommended.

1 ug, 5 ug, 10 ug, 50 ug, 100 ug of active protein