Active Recombinant Human Interleukin-4

C701H1118N216O204S7

0, 551

Endotoxin Level : < 0.04 EU / ug protein (LAL method)

Interleukin-4 (IL-4) is a pleiotropic cytokine that regulates diverse T and B cell responses including differentiation of naive T cells into the TH2 phenotype, promoting B cell proliferation, antibody isotype switching, and expression of other TH2 cytokines including IL-5 and IL-9. IL-4 plays a critical role in the development of allergic inflammation and asthma.It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. IL-4 is produced primarily by activated CD4+ T-lymphocytes, NK 1.1+ T-cells (NKT), mast cells and basophils. One major role of IL-4 is priming the differentiation of precursor CD4+ T-cells into Th2 effector cells by altering their cytokine expression during activation. IL-4 has also been shown to have other stimulatory effects on B-cells including increased expression of CD23, IL-4R, IgM and MHC class II molecules on their surface.

Recombinant human IL-4 is lyophilized from 10mM Phosphate Potasium buffer pH 7.5 and 100 mM NaCl.

Functional studies, Cell assay, SDS-PAGE, Western Blot, Antibody Production

2. Effect of rH Interleukin-4 on kerotinocytes cell proliferation (HaCaT cells).The activity is determined by the dose-dependent stimulation of the proliferation of kerotinocytes cells and the cell viability was assessed by MTT. - ED50 < 1 ng/ml

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Centrifuge vial before opening. Lyophilized protein should be reconstituted in water to a concentration of 100 ng/ul. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. Optimal concentration should be determined for specific application and cell lines.

Room temperature