Active Recombinant Human Interleukin-10

C871H1358N252O252S11

0.390

Endotoxin Level : < 0.04 EU / ug protein (LAL method)

Interleukin 10 (IL-10) is an anti-inflammatory cytokine initially characterized as a T helper (TH)2 specific cytokine, however, further investigations revealed that IL- 10 production was also associated with T regulatory cell responses. It is now known that almost all cells of both the innate and adaptive arms of the immune system can express IL-10, including dendritic cells (DC), macrophages, mast cells, natural killer cells (NK), eosinophil's, neutrophils, B cells, CD8C T cells, and TH1, TH2, and TH17 CD4C T cells IL-10 functions by inhibiting pro-inflammatory cytokines made by macrophages and regulatory T cells including, IFN-gamma, TNF-alpha, IL-2, and IL-3, IL-4, and GM-CSF. IL-10 is also known to suppress antigen presentation on antigen presenting cells. It also stimulates the growth of mast cells, is a cytotoxic T cell differentiation factor, and stimulates B cell differentiation.

Recombinant human IL-10 is lyophilized from a Tris HCl 0.05M buffer at pH 7.4.

Functional studies, Cell culture, Western Blot

-

-

Centrifuge vial before opening. Lyophilized protein should be reconstituted in water to a concentration of 100 ng/ul. Due to the protein nature, dimmers and multimers may be observed. Optimal concentration should be determined for specific application and cell lines.

Room temperature