Hersteller |
American Research Products
|
Kategorie |
|
Typ |
Proteins Recombinant |
Specific against |
other |
Menge |
20 ug |
ArtNr |
ARP-01-2024-3-20 |
eClass 6.1 |
34160400 |
eClass 9.0 |
42020190 |
Lieferbar |
|
Description |
Glutaredoxin(GRX), also known as thioltransferase, is member of the thiol-disulfide oxidoreductase family. Glutraredoxin catalyzes the reversible reduction of protein-glutathionyl mixed disulfides to free sulfhydryl groups though a monothiol mechanism. Glutaredoxin-3 (Grx3/PICOT) is an essential protein involved in the regulation of signal transduction, for instance during immune cell activation and development of cardiac hypertrophy, presumably in response to redox signals. And it has been shown to interact with PRKCQ. Recombinant human Glutaredoxin-3, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
Form |
Liquid. 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol, 1mM DTT |
Storage |
Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles. |
Synonyms |
Thioredoxin-like protein 2, GLRX3, PICOT, TXNL2, GRX3, glutaredoxin 4, bA500G10.4, GLRX4, GRX4, thioredoxin-like 2, thioredoxin-like 2, glutaredoxin 3, |
Concentration |
0.5 mg/ml (determined by Bradford assay) |
NCBI |
NP_006532 |
References |
- Witte S., et al. (2000) J Biol Chem 275(3): 1902-9.
- Haunhorst P., et al. (2010) Biochem Biophys Res Commun. 394(2):372-6
|
Antigen Recognized in Species |
Human |
Purity |
>, 95% by SDS - PAGE |
Molecular Weight |
39.6kDa (355aa), confirmed by MALDI-TOF |
Amino Acid Sequence |
MGSSHHHHHH SSGLVPRGSH MAAGAAEAAV AAVEEVGSAG QFEELLRLKA KSLLVVHFWA PWAPQCAQMN EVMAELAKEL PQVSFVKLEA EGVPEVSEKY EISSVPTFLF FKNSQKIDRL DGAHAPELTK KVQRHASSGS FLPSANEHLK EDLNLRLKKL THAAPCMLFM KGTPQEPRCG FSKQMVEILH KHNIQFSSFD IFSDEEVRQG LKAYSSWPTY PQLYVSGELI GGLDIIKELE ASEELDTICP KAPKLEERLK VLTNKASVML FMKGNKQEAK CGFSKQILEI LNSTGVEYET FDILEDEEVR QGLKAYSNWP TYPQLYVKGE LVGGLDIVKE LKENGELLPI LRGEN |
Gene Category |
Redox Proteins |
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