SensoLyte FDP Alkaline Phosphatase ELISA Assay Kit *Fluorimetric*

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The secondary antibody and streptavidin conjugates of calf intestinal alkaline phosphatase are extensively used as secondary detection reagents in ELISA. The SensoLyte FDP Alkaline Phosphatase ELISA Assay Kit uses highly purified FDP to quantify alkaline phosphatase activity in ELISA. It has been used to detect as low as atto molar (10-15 M) concentration of test antibody in an ELISA format. The kit contains:, FDP phosphatase substrate (Ex/Em=485+/-20/528+/-20 nm upon dephosphorylation) , Assay buffer , Stop solution , Wash buffer , Alkaline phosphatase-conjugated secondary antibody (goat anti-rabbit IgG) , An optimized ELISA protocol