SensoLyte® 520 b-Secretase (BACE1) Activity Assay Kit Fluorimetric - 1 kit

FRET; Protease activity

Beta-Secretase

Dry Ice

BACE1 (beta-Secretase) is a membrane-anchored aspartic protease existing in acidic subcellular vesicles. It is a key player in producing beta-amyloid peptides through proteolytic cleavage of the beta-amyloid precursor protein (APP). Accumulation of neurotoxic beta-amyloid peptide is seen in senile plaques in the brains of patients with Alzheimer's disease (AD), an age-related cognitive disorder. Therefore, targeting beta-secretase is considered a potential therapeutic strategy for the prevention and treatment of AD. The SensoLyte 520 BACE1 (beta-Secretase) Assay Kit is a homogeneous assay that can be used to detect the activity of enzyme and for screening of secretase inhibitors. It contains a QXL 520/ HiLyte Fluor 488 FRET substrate. The sequence of this FRET peptide is derived from the beta-secretase cleavage site on the Swedish APP mutation. This mutation causes the enhancement of beta-secretase to process APP, resulting in an early onset of AD. Active beta-secretase cleaves FRET substrate into two separate fragments resulting in the release of HiLyte Fluor 488 fluorescence that can be monitored at excitation/emission= 490/520 nm. The long wavelength fluorescence of HiLyte Fluor 488 is less interfered by the autofluorescence of components in biological samples and test compounds. Kit Size: 100 assays (96-well plate)

Neuroscience / 0

520

Fluorescent

41105331