SensoLyte® 520 ß-Secretase (BACE1) Activity Assay Kit Fluorimetric - 1 kit

FRET; Protease activity

Beta-Secretase

Store component F at -80°C. Store all other components at -20°C. Component D and E can be stored at room temperature for convenience. Protect components A and B from light and moisture.

?-Secretase (BACE1) is a membrane-anchored aspartic protease existing in acidic subcellular vesicles. It is a key player in producing ?-amyloid peptides through proteolytic cleavage of the ?-amyloid precursor protein (APP). Accumulation of neurotoxic ?-amyloid peptide is seen in senile plaques in the brains of patients with Alzheimer’s disease (AD), an age-related cognitive disorder. Therefore, targeting ?-secretase is considered a potential therapeutic strategy for the prevention and treatment of AD.
The SensoLyte® 520 ?-Secretase Assay Kit is a homogeneous assay that can be used to detect the activity of enzyme and for screening of secretase inhibitors. It contains a QXL® 520/ HiLyte™ Fluor 488 FRET substrate. The sequence of this FRET peptide is derived from the ?-secretase cleavage site on the Swedish APP mutation. This mutation causes the enhancement of ?-secretase to process APP, resulting in an early onset of AD. Active ?-secretase cleaves FRET substrate into two separate fragments resulting in the release of HiLyte™ Fluor 488 fluorescence that can be monitored at excitation/emission= 490/520 nm. The long wavelength fluorescence of HiLyte™ Fluor 488 is less interfered by the autofluorescence of components in biological samples and test compounds.

490

Fluorescent

Research use

5400535133030