SensoLyte® 520 HCV Protease Assay Kit Fluorimetric - 1 kit

Assay Kits

4 °C

The SensoLyte 520 HCV Assay Kit uses a new FRET peptide substrate that incorporates 5-FAM (fluorophore) and QXL 520 (quencher) for a continuous measurement of enzyme activities. In the intact FRET peptide, the fluorescence of 5-FAM is quenched by QXL 520. Upon cleavage of the FRET peptide by HCV NS3/4a protease, the fluorescence of 5-FAM is recovered and can be continuously monitored at excitation/emission = 490 nm/520 nm. With superior fluorescence quantum yield and longer emission wavelength, this 5-FAM/QXL 520-based FRET peptide shows less interference from autofluorescence of test compounds and cellular components. This assay is ten times more sensitive than an EDANS/DABCYL based assay and can detect 0.1 pmole of HCV NS3/4a protease. The assays are performed in a convenient 96-well or 384-well microplate format. The kit contains: - 5-FAM/QXL 520-based FRET peptide substrate (Ex/Em=490/520 nm upon cleavage) - Fluorescence reference standard for calibration - A detailed protocol

Viral & bacterial Proteases / 0

520

Fluorescent

Research use