SensoLyte® 520 MMP-7 Assay Kit Fluorimetric - 1 kit

FRET; Protease activity

Matrix Metalloproteinase (MMP), MMP-7

Store all components at -20°C. Protect components A and B from light. Components D and E can be stored at 4 °C for convenience.

MMP-7 (matrilysin, PUMP) is involved in biological events, such as tissue remodeling, cell proliferation and host defenses.
SensoLyte® 520 MMP-7 Assay Kit uses a 5-FAM (fluorophore) and QXL520™ (quencher) labeled FRET peptide substrates for continuous measurement of the enzyme activities. In an intact FRET peptide, the fluorescence of 5-FAM is quenched by QXL520™. Upon the cleavage of the FRET peptide by MMP-7, the fluorescence of 5-FAM is recovered, and can be continuously monitored at excitation/emission = 490 nm/520 nm. With superior fluorescence quantum yield and longer emission wavelength, 5-FAM/QXL520™ based FRET peptide is less interfered by the autofluorescence of test compounds and cellular components and provides better assay sensitivity.

490

Fluorescent

No

41105331