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AnaTag™ HiLyte™ Fluor 555 Microscale Protein Labeling Kit - 1 kit

ArtNr AS-72046
Hersteller AnaSpec
Menge 1 kit
Kategorie
Typ Kit
Specific against other
Dry ice Yes
ECLASS 10.1 32161090
ECLASS 11.0 32161090
UNSPSC 41116126
Alias Protein labeling kit
Versandbedingung Trockeneis
Lieferbar
Manufacturer - Type
Protein labeling kits
Manufacturer - Applications
Labeling
Manufacturer - Category
Assay Kits - AnaTag / Assay Kits - AnaTag / Labeling & detection / Fluorescent Dye
Shipping Temperature
4 °C
Storage Conditions
Store component A at -20°C. Store all other components at 4°C. Protect component A from light and moisture.
Manufacturer - Research Area
Peptide & Protein Analysis / Protein Labeling Kits
Description
HiLyte™ Fluor 555 acid, SE is an excellent amine-reactive fluorescent labeling dye that generates the protein conjugates that are only slightly red-shifted compared to those of Cy® 3 dyes, resulting in an optimal match to filters designed for Cy® dyes. The HiLyte™ Fluor 555-protein conjugates are very stable and suitable for immunofluorescent staining, in situ hybridization, flow cytometry and other biological applications. Additionally, our in-house data indicated that HiLyte™ Fluor 555 is superior to Cy® 3 in fluorescence polarization-based assays. The kit has all essential components for performing the conjugation reaction and for purifying the HiLyte™ Fluor 555-protein conjugate. The entire process takes only half an hour. The kit is able to perform three conjugation reactions with each reaction labeling up to 200 µg IgG. Kit Size: 3 labeling reactions (3 x 200 µg protein).
Note: AnaTag kits are not intended for peptide labeling.
Kits Components
Component A: HiLyte™ Fluor 555 SE Amino-reactive dye: 3 vials Component B: Reaction buffer for pH adjustment of the conjugation reaction: 0.5 mL Component C: Spin column to purify dye-protein conjugate: 3 pre-packed columns Component D: DMSO solvent for preparing dye stock solution: 150 µL Component E: Elution buffer for eluting dye-protein conjugate: 20 mL Component F: Wash tube to hold buffer for Spin column: 3 tubes Component G: Collect tube to collect dye-protein conjugate: 3 tubes
Absorbtion (nm)
553
Emission (nm)
568
Detection Method
Fluorescent
Feature
Orange (565-620 nm)
Post Synthesis Coupling
No
Usage
Research use
UNSPSC
41105331
GTIN
5400535132149

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 1 kit
Lieferbar: In stock
lieferbar

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