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AnaTag™ HiLyte™ Fluor 488 Microscale Protein Labeling Kit - 1 kit

ArtNr AS-72048
Hersteller AnaSpec
Menge 1 kit
Kategorie
Typ Kit
Specific against other
Dry ice Yes
ECLASS 10.1 32161090
ECLASS 11.0 32161090
UNSPSC 41116126
Alias Protein labeling kit
Versandbedingung Trockeneis
Lieferbar
Manufacturer - Type
Protein labeling kits
Manufacturer - Applications
Labeling
Manufacturer - Category
Assay Kits - AnaTag / Assay Kits - AnaTag / Labeling & detection / Fluorescent Dye
Shipping Temperature
4 °C
Storage Conditions
Store component A at -20°C. Store all other components at 4°C. Protect component A from light and moisture.
Manufacturer - Research Area
Peptide & Protein Analysis / Protein Labeling Kits
Description
HiLyte™ Fluor 488, SE is an excellent amine-reactive fluorescent labeling dye for generating protein conjugates. The spectrum of HiLyte™ Fluor 488 is similar to fluorescein (FITC), resulting in an optimal match to filters designed for fluorescein. HiLyte™ Fluor 488 is more photostable than FITC, providing researchers with additional time for capturing image. Its fluorescence can be detected at the emission wavelength of 523±10 nm when excited at 499±10 nm. The HiLyte™ Fluor 488-protein conjugates are very stable and suitable for immunofluorescent staining, in situ hybridization, flow cytometry and other biological applications. The kit has all essential components for performing the conjugation reaction and for purifying the HiLyte™ Fluor 488-protein conjugate. The entire process takes only half an hour. The kit is able to perform three conjugation reactions with each reaction labeling up to 200 µg IgG. Kit Size: 3 labeling reactions (3 x 200 µg protein).

Note: AnaTag kits are not intended for peptide labeling.
Kits Components
Component A: HiLyte™ Fluor 488 SE Amino-reactive dye: 3 vials Component B: Reaction buffer for pH adjustment of the conjugation reaction: 0.5 mL Component C: Spin column to purify dye-protein conjugate: 3 pre-packed columns Component D: DMSO solvent for preparing dye stock solution: 150 µL Component E: Elution buffer for eluting dye-protein conjugate: 20 mL Component F: Wash tube to hold buffer for Spin column: 3 tubes Component G: Collect tube to collect dye-protein conjugate: 3 tubes
Absorbtion (nm)
499
Emission (nm)
523
Detection Method
Fluorescent
Feature
Green (500 -550 nm)
Post Synthesis Coupling
No
Usage
Research use
UNSPSC
41105331
GTIN
5400535132125

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 1 kit
Lieferbar: In stock
lieferbar

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