Vergleich

AnaTag™ 5 Kit - 1 kit

ArtNr AS-72064
Hersteller AnaSpec
Menge 1 kit
Kategorie
Typ Kit
Specific against other
Dry ice Yes
ECLASS 10.1 32161090
ECLASS 11.0 32161090
UNSPSC 41116126
Alias Protein labeling kit
Versandbedingung Trockeneis
Lieferbar
Manufacturer - Type
Protein labeling kits
Manufacturer - Applications
Labeling
Manufacturer - Category
Assay Kits - AnaTag / Assay Kits - AnaTag / Labeling & detection / Fluorescent Dye
Shipping Temperature
4 °C
Storage Conditions
Store component A at -20°C. Store all other components at 4°C. Protect component A from light and moisture.
Manufacturer - Research Area
Peptide & Protein Analysis / Protein Labeling Kits
Description
TAMRA is one of the most popular fluorophores used in various bioconjugations. 5-TAMRA is the purified single isomer of 5(6)-TAMRA. It is preferred for some complicated biological applications where reproducibility is more critical than material cost since the minor positional difference between 5-TAMRA and 6-TAMRA might affect some biological properties of the underlying conjugates. 5-TAMRA is more often used than 6-TAMRA for labeling peptides and proteins. 6-TAMRA is predominately used for labeling nucleotides and sequencing nucleic acids. The 5-TAMRA-protein conjugates are suitable for immunofluorescent staining, in situ hybridization, flow cytometry and other biological applications. The kit has all essential components for performing the conjugation reaction and for purifying the 5-TAMRA-protein conjugates. The kit is able to perform three conjugation reactions with each reaction labeling up to 200 µg IgG.
Note: AnaTag kits are not intended for peptide labeling.
Kits Components
Component A: 5-TAMRA SE Amino-reactive dye: 3 vials Component B: Reaction buffer for pH adjustment of the conjugation reaction: 0.5 mL Component C: Spin column to purify dye-protein conjugate: 3 pre-packed columns Component D: DMSO solvent for preparing dye stock solution: 150 µL Component E: Elution buffer for eluting dye-protein conjugate: 20 mL Component F: Wash tube to hold buffer for Spin column: 3 tubes Component G: Collect tube to collect dye-protein conjugate: 3 tubes
Absorbtion (nm)
547
Emission (nm)
574
Unit Definition
One unit of protease hydrolyzes 1 picomole of Mca-Arg-Pro-Lys-Pro-Val-GluNva-Trp-Arg-Lys(Dnp)-NH2 per minute at pH 7.5 at 25°C
Detection Method
Fluorescent
Feature
Orange (565-620 nm)
Post Synthesis Coupling
No
Usage
Research use
UNSPSC
41105331
GTIN
5400535131982

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 1 kit
Lieferbar: In stock
lieferbar

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