SensoLyte® ONPG ß-Galactosidase Assay Kit Colorimetric - 1 kit

Enzyme activity

Beta-Galactosidase

Store all components at -20°C. Components C and D can be stored at room temperature for convenience. Protect component A from light.

?-galactosidase, encoded by the lacZ gene in E. coli, is widely used as a reporter enzyme to study gene expression, protein-protein interaction and normalization of transfection efficiency in mammalian cells. The SensoLyte® ONPG ?-Galactosidase Assay Kit utilizes the widely used chromogenic substrate, o-nitrophenyl ?-D-galactopyranoside (ONPG) to determine the activity of ?-galactosidase in transfected cells or tissues. In the presence of ?-galactosidase, the colorless compound is hydrolyzed into o-nitrophenol, producing a yellow color that can be detected at absorbance 420 nm. The intensity of the color produced is proportional to ?-galactosidase activity.

420

1 ng/ml

Research use

5400535131395