SensoLyte® Thiol Quantitation Assay Kit “Colorimetric” - 1 kit

Assay Kits

4 °C

Thiols, compounds that contain sulfhydryl group, are powerful reducing agents capable of acting as antioxidants, enzyme cofactors, and neuromodulators. The SensoLyte Thiol Quantitation Assay Kit utilizes the widely used Elmann's reagent for colorimetric measurement of thiol concentrations. The sulfhydryl group of thiol reacts with Ellman's Reagent [5, 5'-dithiobis (2-nitrobenzoic acid)] and generates 2-nitro-5-thiobenzoic acid (TNB) producing a yellow color that can be detected at absorbance 420 nm. The intensity of the color produced is proportional to thiol concentration. Kit Size: 500 assays (96-well plate).

Cell Biology & Protein Analysis; Peptide & Protein Analysis / 0

5400535131388

1.5 uM

41105333