SensoLyte® Thiol Quantitation Assay Kit “Colorimetric” - 1 kit

Analyte quantitation

Thiol

Store all components at -20°C. Component C can be stored at room temperature for convenience. Protect component A from light and moisture.

Thiols, compounds that contain sulfhydryl group, are powerful reducing agents capable of acting as antioxidants, enzyme cofactors, and neuromodulators. The SensoLyte® Thiol Quantitation Assay Kit utilizes the widely used Elmann’s reagent for colorimetric measurement of thiol concentrations. The sulfhydryl group of thiol reacts with Ellman’s Reagent [5, 5’-dithiobis (2-nitrobenzoic acid)] and generates 2-nitro-5-thiobenzoic acid (TNB) producing a yellow color that can be detected at absorbance 420 nm. The intensity of the color produced is proportional to thiol concentration.

420

1.5 uM

Research use

5400535131388