SensoLyte® 440 Cathepsin B Assay Kit Fluorimetric - 1 kit

FRET; Protease activity

Cathepsin, Cathepsin B

Store component C at -80°C. Store all other components at -20°C. Component D can be stored at room temperature for convenience. Protect components A and B from light and moisture.

Cathepsins are a class of globular lysosomal proteases playing a vital role in mammalian cellular turnover. They degrade polypeptides and are distinguished by their substrate specificities. The cathepsin family consists of 12 cysteine proteases with broad exo- and endopeptidase activity, of which Cathepsin B is a member. Elevated levels of cathepsin B were detected in metastases and neurological disorders including Alzheimer's disease (AD).
The SensoLyte® 440 Cathepsin B Assay Kit provides a fluorogenic peptide for measurement of enzyme activity. This peptide releases the AMC (7-amino-4-methylcoumarin) fluorophore upon cathepsin B cleavage and can be detected with excitation at 354 nm and emission at 442 nm.

354

Fluorescent

Research use

5400535131081