SensoLyte® 520 TEV Protease Assay Kit Fluorimetric - 1 kit

FRET; Protease activity

Tobacco Etch Virus (TEV)

Store component C at -80°C. Store all other components at -20°C. Component D can be stored at room temperature for convenience. Protect Components A and B from light and moisture.

Tobacco Etch Virus (TEV) proteinase is widely used to remove fusion tags from recombinant proteins. It recognizes specific sequence Glu-Asn-Leu-Tyr-Phe-Gln-Gly and cleaves between Glutamine and Glycine residues. TEV protease is active even at 4 °C, making it the protease of choice for fusion proteins or tags removal.
The SensoLyte® TEV Protease Assay Kit uses TEV specific Glu-Asn-Leu-Tyr-Phe-Gln-Gly substrate labeled with 5-FAM dye and QXL™ 520 quencher. Proteolytic cleavage of this quenched 5-FAM-QXL™ 520 conjugate yields a brightly green fluorescence, which can be continuously monitored at Ex/Em= 490/520 nm. Increase in fluorescence intensity is directly
proportional to the TEV protease quantity/activity.

490

Fluorescent

Research use

5400535130206