SensoLyte® 520 Meprin a Activity Assay Kit Fluorimetric - 1 kit

Assay Kits

Mammalian Cells

Dry Ice

Meprin alpha can cleave extracellular matrix proteins (pro-collagen I, fibronectin, osteopontin, SPARC), growth factors (VEGF-A, CTGF) and proteinases (MMP-1). Evidence has suggested that Meprin alpha is associated with cancer proliferation, cancer migration and invasion. The FRET substrate included in SensoLyte 520 Meprin alpha Activity Assay Kit was designed to detect Meprin alpha activity and reduce the cross reactivity with Meprin beta, ADAM10, alpha -secretase, BACE-2, ECEs and TACE. This assay kit can be used to detect enzyme activity in purified enzyme preparations and compound screening. Active Meprin alpha cleaves the FRET substrate, resulting in an increase of 5-FAM fluorescence, monitored at excitation/emission = 490 nm/520 nm. The long wavelength fluorescence of 5-FAM is also less interfered by the autofluorescence of components in biological samples and test compounds. This assay can detect as low as 0.1 ng/mL active Meprin alpha. The kit contains: - 5-FAM/QXL 520 Meprin alpha substrate, Ex/Em=490/520 nm upon cleavage - Assay buffer - Recombinant Human Meprin alpha - Meprin alpha activation buffer - Meprin alpha stop solution - Inhibitor - Fluorescence reference standard for calibration - A detailed protocol Kit size: 100 assays (96-well plate) Figure 1. Inhibition of Meprin alpha activity by Actinonin as measured with SensoLyte 520 Meprin alpha Activity Assay Kit.

Adhesion & Extracellular Matrix; Cancer & Apoptosis / 0

520

Fluorescent

Research use