SensoLyte® 520 Meprin a Activity Assay Kit Fluorimetric - 1 kit

FRET; Protease activity

Meprin

NSO (mammalian cells)

Store component C at -80°C. Store all other components at -20°C. Protect components A and B from light and moisture. Component F can be stored at room temperature for convenience.

Meprin ? is a zinc endopeptidase of the astacin family and the metzincin superfamily. Meprin ? can cleave extracellular matrix proteins (pro-collagen I, fibronectin, osteopontin , SPARC), growth factors (VEGF-A, CTGF) and proteinases (MMP-1). Evidence has suggested that Meprin ? is associated with cancer proliferation, cancer migration and invasion.
The FRET substrate included in SensoLyte® 520 Meprin ? Activity Assay Kit was designed to detect Meprin ? activity and reduce the cross reactivity with Meprin ?, ADAM10, ?-secretase, BACE-2, ECEs and TACE. This assay kit can be used to detect enzyme activity in purified enzyme preparations and compound screening. Active Meprin ? cleaves the FRET substrate, resulting in an increase of 5-FAM fluorescence, monitored at excitation/emission = 490 nm/520 nm. The long wavelength fluorescence of 5-FAM is also less interfered by the autofluorescence of components in biological samples and test compounds. This assay can detect as low as 0.1 ng/mL active Meprin ?.

490

Fluorescent

No

41105331