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Protein A-HiLyte™ Fluor 750 Conjugate

ArtNr AS-72239
Hersteller AnaSpec
Menge 1 mg
Kategorie
Typ Proteins Recombinant
Format Liquid
Applikationen WB, IF, IP, IHC
Specific against other
Host E.coli
Konjugat/Tag Unconjugated
Purity >95% by SDS-PAGE
Dry ice Yes
Citations 1. Björk, I. Eur J Biochem 29 (1972):579-584. 2. Sjödahl, J. Eur J Biochem 78 (1977): 471-490.3. Schröder, A. K. et al. Immunol 57 (1985): 305-309.4. Rosenberg, I.M. Protein Analysis and Purification, Birkhäuser, 2005: 41-47.
ECLASS 10.1 32160409
ECLASS 11.0 32160409
UNSPSC 12352202
Alias Protein A
Versandbedingung Trockeneis
Lieferbar
Manufacturer - Type
Labeled proteins
Manufacturer - Applications
Immunohistochemistry (IHC); Immunofluorescence (IF); Immunoprecipitation (IP); Western Blotting
Manufacturer - Category
Dyes & Reagents Group 3 / Dyes & Reagents Gro / Labeling & detection / Proteins / IR (>750 nm)
Manufacturer - Targets
Protein A
Shipping Temperature
4 °C
Storage Conditions
Stable for 2-3 months at 4°C. For long-term storage, divide the solution into aliquots and store at -20°C or add an equal volume of glycerol and store the solution at -20°C without aliquoting. Avoid multiple thaw-freeze cycles.
Molecular Weight
~30000
Manufacturer - Research Area
Peptide & Protein Analysis / Labeling Reagents
Description
Protein A is a non-glycosylated cell wall protein of Staphylococcus aureus that can bind Fc part of immunoglobulin molecule of different species with strong affinity. Protein A consists of five IgG binding domains (A, B, C, D, and E), each is approximately 60 amino acids long with no cysteines present and two Staphylococcus aureus cell membrane binding domains (X and M).
AnaSpec's recombinant Protein A consists of only IgG binding domains and was expressed in E.coli. Its apparent molecular mass is approximately 30, 000 Da compared to the most reported 42, 000 Da for the native Protein A that is found in Staphylococcus aureus. Recombinant Protein A conjugated with HiLyte™ Fluor-750 can be used for immunoglobulin detection in IHC and IF and for assay development. Near-infrared fluorescence. Excitation/Emission wavelength= 754 nm/ 778 nm
Absorbtion (nm)
754
Emission (nm)
778
Colour
Infrared
Concentration
1 mg/mL
Storage Buffer
10mM phosphate, 150mM sodium chloride, pH 7.2, 0.1% sodium azide as preservative
Detection Method
Fluorescent
Post Synthesis Coupling
No
Usage
Research use
UNSPSC
12352202
GTIN
5400535130046

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 1 mg
Lieferbar: In stock
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